青藤碱对子宫内膜异位症大鼠异位组织TNF-α和NF-κB水平的影响

Effect of sinomenine on tumor necrosis factor-α and nuclear factor-κB in the heterotopic tissue in rats with endometriosis

目的研究青藤碱对子宫内膜异位症模型大鼠异位组织中肿瘤坏死因子(TNF-α)和核转录因子(NF-κB)水平的影响。方法实验分为青藤碱灌胃组、空白组、模型组、丹那唑组,采用免疫组化法观察子宫内膜异位症模型大鼠异位组织中TNF-α和NF-κB水平;光学显微镜下观察各组子宫内膜形态和肉眼观察异位病灶的形态。结果与模型组比较,青藤碱、丹那唑治疗组均能降低子宫内膜异位症大鼠异位组织中TNF-α和NF-κB水平(P<0.05);青藤碱灌胃组异位病灶大小较丹那唑组明显减小(P<0.05)。结论青藤碱通过降低参与子宫内膜的黏附种植一血管生成-侵润发展3个阶段的细胞因子水平及活性,抑制子宫内膜细胞的黏附、种植和进一步生长。

Objective To investigate the effect of sinomenine on the level of tumor necrosis factor-α （TNF-α） and nuclear factor-κB （NF-κB） and in the heterotopic tissue in rats with endometriosis. Methods The rats with endometriosis were divided into sinomenine lavage group, blank control group, model group and dannazol group, and the levels of TNF-α and NF-κB in the heterotopic tissues of the rats were detected with immunohistochemistry. Results Sinomenine lavage and dannazol treatment both significantly decreased the levels of levels of TNF-α and NF-κB in the heterotopic tissues of the rats as compared with the model group （P〈0.05）, and lesions were significantly smaller in sinomenine lavage group than in dannazol group. Conclusion Sinomenine can inhibit the production and activity of TNF-α and NF-κB to suppress the adhesion, implantation, infiltration and growth of the endometrial cells in the rat model of endometriosis.