大鼠衰老过程中大脑运动皮质内GLUT3的表达

Expression of glucose transporter-3 in the cerebral cortex of aging rats

目的观察不同月龄大鼠大脑运动皮质内GLUT3的表达规律。方法用免疫组织化学染色方法测定不同月龄组(3、18、24、30月组)大鼠大脑运动皮质内GLUT3的表达情况。结果大鼠大脑运动皮质内GLUT3的表达随月龄增加呈抛物线改变。由3月组至18月组,GLUT3的含量随月龄增加而增加(P<0.01);由18月组至30月,GLUT3的含量随月龄增加而减少(P<0.01)。结论大鼠大脑运动皮质内GLUT3的表达随月龄增加呈抛物线改变,中年期以前呈增加趋势,中年期以后呈减弱趋势,提示GLUT3在神经系统发育成熟过程和神经系统衰老过程中发挥一定的作用。

Objective To observe the changes in the expression of glucose transporter-3 （GLUT3） in the cerebral cortex of rats during aging and investigate the role of GLUT3 in the aging process of the nervous system. Methods The cerebral tissues were collectd from rats of 3, 18, 24, and 30 months old （10 in each age group）, and the expression of GLUT3 in the cerebral cortex was detected by immunohistochemistry. Results Under optical microscope, GLUT3-positive cells were found in every group. Within the age range of 3 to 8 months, GLUT3-positive cells increased significantly with age （P〈0.01）, but at 24-30 months of age, the number of GLUT3-positive cells reduced significant with age （P〈0.01）. Conclusion The expression changes of GLUT3 ir the cerebral cortex of rats during aging indicate that GLUT3 plays an important role in the maturation and aging of the nervous system.