TOP2A在结直肠癌组织中的表达和意义

Expression of topoisomerase II alpha in human colorectal carcinoma and its significance

目的检测TOP2A在结直肠癌、癌旁组织及转移癌组织中的表达,初步探讨其与结直肠癌发生发展、侵袭转移关系。方法用免疫组化及荧光定量PCR方法检测结直肠癌、淋巴结转移癌及相应癌旁组织中TOP2A在蛋白及转录水平的表达情况。结果 TOP2A蛋白在淋巴结转移癌中的表达高于原发灶及相应正常癌旁组织中的表达(P<0.05),在结直肠癌组织中TOP2A的表达与癌组织的浸润程度、淋巴结转移密切相关(P<0.05),癌组织与相应的癌旁组织相比差异则无显著性(P>0.05),其表达与癌组织分化程度也无显著性(P>0.05),在mRNA水平的检测也得到了与上述结果基本一致的结论。结论 TOP2A的表达与结直肠癌侵袭转移关系较密切,其表达水平在一定程度上提示结直肠癌的转移潜能,检测TOP2A蛋白可作为结直肠癌诊断、治疗及预后判断过程中的一种较有价值的病理学指标。

Objective To detect the correlation between the expression of topoisomerase 2 alpha （TOP2A） and the biological behaviors of human colorectal carcinoma. Methods Immunohistochemistry and real-time RT-PCR were used to detect the expression of TOP2A in colorectal carcinomas and normal mucosa. Results The protein and mRNA expressions of TOP2A in the metastatic lymph nodes were significantly higher than those in matched primary lesions and normal tissues （P〈0.05）. No significant difference was found in TOP2A expressions between normal mucosa and colorectal carcinomas. The protein and mRNA expressions of TOP2A were significantly correlated to the lymph node metastasis and invasion depth （P〈0.05）, but not to the differentiation of the tumor （P〉0.05）. Conclusion TOP2A plays an important role in the invasion and metastasis of the colorectal carcinomas, and may serve as a valuable indicator for the diagnosis, treatment and the prognostic evaluation of the malignancy.