Bcl-2基因在鼠T-细胞淋巴瘤细胞中抗凋亡作用的实验研究

An experimental study on Bcl-2 gene related to anti-apoptosis in murine T-cell lymphoma

目的应用转染了Ｂｃｌ－２基因的鼠Ｔ－细胞淋巴瘤ＷＨｂ１２细胞系进一步研究Ｂｃｌ－２基因的抗凋亡作用及意义。方法分别采用紫外线照射２０ｓ、１０μｍ地塞米松作用２４ｈ和２００ｎｇ／ｍｌ鬼臼乙叉甙作用２４ｈ等方法，诱导ＷＨｂ１２细胞和其亲本细胞Ｗ７２ＵＳＡ细胞凋亡，与亲本细胞相比较，观察转染了Ｂｃｌ－２基因的ＷＨｂ１２细胞是否具有抗凋亡作用。结果未转染Ｂｃｌ－２基因的亲本细胞Ｗ７２ＵＳＡ细胞发生了凋亡，而转染了Ｂｃｌ－２基因的ＷＨｂ１２细胞存活完好，无明显凋亡发生。结论进一步证实了Ｂｃｌ－２基因具有显著的抗凋亡作用，其抗凋亡作用谱广，具有重要意义。

Objective The effect and significance of Bcl-2 gene on apoptosis were investigated using WHb 12 cell line with transferred Bcl-2 gene from murine T-cell lymphoma.Methods The apoptosis was induced by UV radiation for 20 seconds,10 μm dexamethasone and 200 ng/ml etopside for 24 hours separately,in the WHb 12 cells and their parental W 7.2 USA cells for comparison to observe whether the WHb 12 cells with transferred Bcl-2 gene are the apoptotic blocker.Results It is showed that the apoptosis occurr in the parental W 7.2 USA cells without transfererd Bcl-2 gene,whereas it did not occurred in the WHb 12 cells with transferred Bcl-2 gene,which survived very well.Conclusion This result comfirmed that Bcl-2 gene is a powerful and broad spectrum blocker of apoptosis.