鸭疫里氏杆菌敏感烈性噬菌体RAP44的生物学特性

Biological properties of the virulent bacteriophage RAP44 sensitive to Riemerella anatipestifer

为了解鸭疫里氏杆菌烈性噬菌体RAP44的临床应用潜力,按常规方法对其生物学特性进行了测定。结果显示,该噬菌体在pH5～pH9的环境中稳定;55℃以下作用30min仍可保持较高裂解活性;加入15mmol/L的Mg2+或20mmol/L的Ca2+后噬菌体RAP44的出斑率分别提高61.9%和53.8%;最佳感染复数为1/40;潜伏期为10min,裂解期为40min,裂解量为83;SDS-PAGE分析结果显示,有2种主要的衣壳蛋白,分子质量分别为40ku和17ku;其基因组约为40kb。表明,噬菌体RAP44潜伏期短,裂解量大,可作为生物防治鸭疫里氏杆菌的候选噬菌体株。

To explore the potential clinical application of the virulent bacteriophage RAP44 sensitive to Riemerella anatipestifer,the biological properties of the phage were detected by the conventional method.It had relatively high stability from pH 5 to pH 9.It still remained high lysis activity after being incubated at 55 ℃ for 30 min.After 15 mmol/L Mg^2＋ or 20 mmol/L Ca^2＋ were added to the medium,the numbers of plaques were 61.9% and 53.8% higher than into the medium without Mg^2＋ or Ca^2＋ respectively.The optimal multiplicity of infection was 0.025.The latent period was about 10 min,burst period was 40 min,and the average burst size was about 83 PFU/cell.SDS-PAGE profiles indicated that RAP44 contained two capsid proteins,and the two capsid proteins were 40 ku and 17 ku in molecular weight respectively.The genome of the phage was about 40 kb in size.The latent period of phage RAP44 was short and the average burst size was great,indicating that it could be used as a candidate phage strain for biological control R.anatipestifer.