维生素E影响肿瘤坏死因子-α对贮脂细胞增殖及胶原合成的作用

Effect of Vitamin E on the Proliferation of Rat FatStoring Cells and Their Collagen Synthesis

目的：研究维生素Ｅ（ＶｉｔＥ）对大鼠贮脂细胞增殖及胶原合成的影响。方法：以链酶蛋白酶和胶原酶液原位循环灌注法分离大鼠贮脂细胞，以肿瘤坏死因子－α作为刺激因素，采用３Ｈ－胸腺嘧啶核苷（３Ｈ－ＴｄＲ）和３Ｈ－脯氨酸（３Ｈ－ｐｒｏｌｉｎｅ）掺入法分别测定ＶｉｔＥ对贮脂细胞增殖和胶原合成的影响，并在倒置显微镜下观察贮脂细胞的形态。结果：（１）肿瘤坏死因子－α能明显促进贮脂细胞增殖，并能明显抑制其胶原合成；（２）ＶｉｔＥ能显著抑制肿瘤坏死因子－α引起的贮脂细胞增殖，并能增强肿瘤坏死因子－α对贮脂细胞胶原合成的抑制作用。结论：ＶｉｔＥ对贮脂细胞增殖及胶原合成具有抑制作用，这可能是ＶｉｔＥ抗肝纤维化作用的重要机制之一。

Objective: This experiment was designed to study the effect of vitamin E on the proliferation and collagen synthesis of rat fatstoring cells. Methods: Fatstoring cells were isolated from a male SprayweDawley rat by modified Friedman's method. The cells were treated with tumor necrosis factor,and the effect of vitamin E on their proliferation and collagen synthesis were studied with 3Hthymidine and 3Hproline incorporation assay. And then they were observed under contrary phase microscopy. Results: 1) The proliferation of fatstoring cells were increased by adding tumor necrosis factor.But the collagen synthesis of the cells were decreased.2) Vitamin E has a markedly inhibitive effect on the abilities of proliferation and collagen synthesis in the cells treated with tumor necrosis factor. Conclusion: Vitamin E inhibits the proliferation and collagen synthesis of fatstoring cells. It is possible that vitamin E affect liver fibrosis by these effects.Perhaps,it is one of the important mechanism links through which the liver fibrosis is inhibited.