摘要
目的研究含人胚胎骨髓基质细胞层体系对脐血造血干/祖细胞的扩增效果。方法分别建立人胚胎骨髓基质细胞层(FBMSCL)和成人骨髓基质细胞层(ABMSCL),观察他们单独及其与造血生长因子(HGFs)联合,在不同时间(第10、21、30、38天)对脐血CD+34细胞、CD+34CD-38细胞、粒单系祖细胞(CFUGM),多向祖细胞(CFUGEMM),早期红系祖细胞(BFUE)的扩增作用。结果(1)人胚胎骨髓基质细胞(FBMSC)单独作用及其与HGFs[包括脐血浆、干细胞因子(SCF)、IL3、IL6、白细胞介素1β(IL1β),促红细胞生成素(EPO)、粒细胞刺激因子(GCSF)]联合作用于CD+34细胞、集落形成细胞(CFCs),其扩增效果均优于成人骨髓基质细胞(ABMSC)。(2)FBMSC+HGFs在其扩增高峰(第30天)所扩增的早中期祖细胞的百分含量明显高于ABMSC+HGFs在其扩增高峰(第30天)所扩增的早中期祖细胞百分含量。(3)FBMSC+HGFs扩增CD+34CD-38细胞达3倍,ABMSC+HGFs对CD+34CD-38细胞数量起维持作用,当扩增到第5~6周,CD+34CD-38?
Objective To study the expansion effect of human fetal bone marrow cells system on the hematopoietic stem cells in umbilical cord blood Methods Fetal bone marrow stromal cell layers (FBMSCL) and adult bone marrow stromal cell layers (ABMSCL) were established and their single and combined effects with hematopoietic growth factors (HGFs) on expansion of colony forming cells (CFCs), CD+34 cells and CD+34CD-38 cells in umbilical cord blood were observed at different time (day 10,21,30,38) Results (1) The expansion effect of FBMSCL on the CD+34 Cells and CFCs was better than ABMSCLs and was also better than ABMSCs when they were combined with HGFs (including umbilical cord blood plasma, SCF, IL3, IL6, IL1 , EPO, GCSF), respectively (2) During the CFCs peak on day 30, the percentage of early and midterm progenitors expanded by FBMSCL+HGFs was higher than that expanded by ABMSCL+HGFs (3) The CD+34CD-38 cells were three folds expanded by FBMSCL+HGFs and were not changed significantly by ABMSCL+HGFs When they were expanded on the fifth to sixth week, CD+34CD-38 cells were not wasted up There were 75% and 25% CD+34CD-38 cells supported by FBMSCL+HGFs and ABMSCL+HGFs, respectively Conclusion Human fetal bone marrow stromal cells system is one of the ideal expansion systems of hematopoietic stem/progenitor cells in umbilical cord blood
出处
《中华儿科杂志》
CAS
CSCD
北大核心
1999年第7期429-433,共5页
Chinese Journal of Pediatrics
基金
美国中国医学基金(CMB)资助
关键词
骨髓细胞
造血干细胞
贫血
FANCONI贫血
one marrow cellsHematopoietic cell growth factorsHematopoietic stem cells