含人胚胎骨髓基质细胞层体系对脐血造血干/祖细胞的扩增效果

The effect of human fetal bone marrow stromal cells on the expansion of hematopoietic stem cells/progenitor cells in umbilical cord blood

目的研究含人胚胎骨髓基质细胞层体系对脐血造血干／祖细胞的扩增效果。方法分别建立人胚胎骨髓基质细胞层（ＦＢＭＳＣＬ）和成人骨髓基质细胞层（ＡＢＭＳＣＬ），观察他们单独及其与造血生长因子（ＨＧＦｓ）联合，在不同时间（第１０、２１、３０、３８天）对脐血ＣＤ＋３４细胞、ＣＤ＋３４ＣＤ－３８细胞、粒单系祖细胞（ＣＦＵＧＭ），多向祖细胞（ＣＦＵＧＥＭＭ），早期红系祖细胞（ＢＦＵＥ）的扩增作用。结果（１）人胚胎骨髓基质细胞（ＦＢＭＳＣ）单独作用及其与ＨＧＦｓ［包括脐血浆、干细胞因子（ＳＣＦ）、ＩＬ３、ＩＬ６、白细胞介素１β（ＩＬ１β），促红细胞生成素（ＥＰＯ）、粒细胞刺激因子（ＧＣＳＦ）］联合作用于ＣＤ＋３４细胞、集落形成细胞（ＣＦＣｓ），其扩增效果均优于成人骨髓基质细胞（ＡＢＭＳＣ）。（２）ＦＢＭＳＣ＋ＨＧＦｓ在其扩增高峰（第３０天）所扩增的早中期祖细胞的百分含量明显高于ＡＢＭＳＣ＋ＨＧＦｓ在其扩增高峰（第３０天）所扩增的早中期祖细胞百分含量。（３）ＦＢＭＳＣ＋ＨＧＦｓ扩增ＣＤ＋３４ＣＤ－３８细胞达３倍，ＡＢＭＳＣ＋ＨＧＦｓ对ＣＤ＋３４ＣＤ－３８细胞数量起维持作用，当扩增到第５～６周，ＣＤ＋３４ＣＤ－３８?

Objective To study the expansion effect of human fetal bone marrow cells system on the hematopoietic stem cells in umbilical cord blood Methods Fetal bone marrow stromal cell layers (FBMSCL) and adult bone marrow stromal cell layers (ABMSCL) were established and their single and combined effects with hematopoietic growth factors (HGFs) on expansion of colony forming cells (CFCs), CD+34 cells and CD+34CD-38 cells in umbilical cord blood were observed at different time (day 10,21,30,38) Results (1) The expansion effect of FBMSCL on the CD+34 Cells and CFCs was better than ABMSCLs and was also better than ABMSCs when they were combined with HGFs (including umbilical cord blood plasma, SCF, IL3, IL6, IL1 , EPO, GCSF), respectively (2) During the CFCs peak on day 30, the percentage of early and midterm progenitors expanded by FBMSCL+HGFs was higher than that expanded by ABMSCL+HGFs (3) The CD+34CD-38 cells were three folds expanded by FBMSCL+HGFs and were not changed significantly by ABMSCL+HGFs When they were expanded on the fifth to sixth week, CD+34CD-38 cells were not wasted up There were 75% and 25% CD+34CD-38 cells supported by FBMSCL+HGFs and ABMSCL+HGFs, respectively Conclusion Human fetal bone marrow stromal cells system is one of the ideal expansion systems of hematopoietic stem/progenitor cells in umbilical cord blood