超临界二氧化碳抗溶剂法制备玉米蛋白纳米颗粒

Preparation of zein nanoparticles by supercritical CO_2 anti-solvent precipitation

采用超临界二氧化碳抗溶剂法从玉米蛋白的丙酮-二甲亚砜混合溶液中制得了玉米蛋白纳米颗粒,考察了温度、压力、溶液浓度、二氧化碳流速和溶液流速对玉米蛋白空白微球形貌和粒径的影响。用扫描电镜、纳米粒度分析、热重分析、差热分析等手段对结果进行表征。实验结果表明,超临界CO2抗溶剂法可用于制备球形度较好、粒径分布较窄的玉米蛋白微球,所得玉米蛋白微球平均粒径在160～400nm之间。温度、压力、溶液浓度都会影响微粒的粒径,选取不同的工艺组合可制备不同粒径及分布要求的微粒。热重分析和差热分析的结果显示,制得的玉米蛋白空白微球较原料稳定性有所提高。

Zein nanoparticles was prepared from the acetone-DMSO mixed solution of zein using the supercritical CO2 anti-solvent precipitation（SAS）.The effects of temperature,pressure,zein solution concentration,flow rate of supercritical CO2 and solution were studied.SEM,Particle Size Analyzer,TGA,DSC were used to characterize the products.The result showed that the zein nanoparticles with good sphericity and narrow size distribution could be prepared by SAS precipitation,and the mean diameters of the microspheres ranging between 160nm and 400nm could be obtained.Temperature,pressure and the concentration of the solution can influence the size of products,so the different size and distribution requirements of zein nanoparticles can be prepared through the selection of different process combinations.The TG analysis curves and DSC thermogram showed that the blank zein nanoparticles were more stable than the law zein.