富含纤溶酶豆豉冻干粉的消化性质及抗血栓作用

Digestive properties and antithrombotic effect of fibrinolytic enzyme-rich Douchi vacuum freeze-dried powder

为研究富含纤溶酶豆豉冻干粉口服的抗血栓作用,以豆豉冻干粉提取液酶活的变化测定模拟胃肠液对该冻干粉的影响,以血块的溶解率测定冻干粉的体外抗血栓作用,并初步确定其中的抗血栓物质。同时还测定了该冻干粉连续灌胃对大鼠凝血系统的影响。结果表明,该冻干粉受模拟胃液影响较大,酶活残留56·21%,在模拟肠液中较稳定,残留酶活大于82·86%。5%豆豉冻干粉提取液对血块的溶解率达40·84%±3·76%,而经热处理的提取液仅为12·48%±2·43%。该冻干粉能延长大鼠血浆凝血酶时间(TT),对活化部分凝血活酶时间(APTT)及凝血酶原时间(PT)无影响。因此,富含纤溶酶豆豉冻干粉宜制成肠溶制剂,具有体内外抗血栓作用,其抗血栓物质主要为豆豉纤溶酶。

To determine the antithrombotic effect of fibrinolytic enzyme-rich Douchi vacuum freeze-dried powder by oral administration,the enzyme activity of Douchi freeze-dried powder extract was measured to observe the effect of simulated gastric and intestinal fluid on the powder.The dissolution rate on blood clots was measured to observe the antithrombotic effect of the powder in vitro,through which the antithrombotic matter in it was primarily determined.The effect of the powder by continuous intragastric administration on the coagulation system in rats was also evaluated.The results showed that,the powder was greatly affected by simulated gastric fluid,the enzyme activity of which decreased to 56.21%,it was more stable in the simulated intestinal fluid,with remaining enzyme activity more than 82.86%.The dissolution rate of 5% Douchi freeze-dried powder extract on blood clots was 40.84%±3.76%,but it was only 12.48%±2.43% with heated extract.Thrombin time（TT）in rat serum could be prolonged by the powder,but activated partial thromboplastin time（APTT）and prothrombin time（PT）were not affected.In conclusion,the fibrinolytic enzyme-rich Douchi freeze-dried powder was suitable to be made into intestinal dissolving form.In vitro and in vivo antithrombotic effect of the powder was observed,with Douchi fibrinolytic enzyme as primary antithrombotic matter in it.