大蒜新素对分离大鼠脑细胞内游离Ca^(2+)的影响<英文>

Effects of allitridi on intracellular Ca^(2+) concentration in isolated rat brain cells

目的:观察大蒜新素对不同刺激剂所致分离大鼠脑细胞内游离钙的影响。方法:以Fura 2-AM为细胞内游离钙的荧光指示剂,用AR-CM-MIC阳离子测定系统,直接测定了分离新生大鼠脑细胞内游离钙([Ca^(2+)]_i)值,观察了大蒜新素的影响。结果:大蒜新素对脑细胞静息[Ca^(2+)]_i无明显影响,大蒜新素1-100μmol·L^(-1)能剂量依赖性地抑制高K^+和谷氨酸引起的[Ca^(2+)]_i升高,其中IC_(50)分别为59.7和69.9μmol·L^(-1),高剂量大蒜新素100μmol·L^(-1)能抑制去甲肾上腺素引起的[Ca^(2+)]_i升高。结论:大蒜新素对高K^+、去甲肾上腺素及谷氨酸引起的[Ca^(2+)]_i升高的抑制作用可能是其抗脑缺血作用机制之一。

AIM: To study actions of allitridi extracted from garlic on intracellular calcium in isolated rat brain cells. METHODS: Brain cells were isolated from newborn rat brain with Fura 2-AM measurements of intracellular Ca2 + concentration ([ Ca2 + ] i). RESULTS: Allitridi 1 - 100 μmol· L-1 concentration-dependently blocked increases of [ Ca2 + ]i caused by potassium chloride and sodium glutamate (Glu) with IC50 of 59.7 and 69.9 μmol· L-1 respectively. Allitridi 100 μmol·L-1 blocked norepinephrine ( Nor )-induced [ Ca2+ ]i elevation. CONCLUSION: Allitridi is an effective agent for blocking the [Ca2+ ]i increase caused by potassium chloride, Nor and Glu.