乙酰胆碱对人子宫颈平滑肌细胞合成肌醇1,4,5三磷酸的影响

Effects of acetylcholine on inositol 1,4,5- triphosphate formation in cervix uteri myo- metrial cells of human

目的:检测乙酰胆碱(acetylcholine,ACh)对人妊娠与未妊娠子宫颈平滑肌细胞生物合成肌醇1,4,5三磷酸(inositol 1,4,5-triphosphate,IP_3)的影响。方法:[~3H]IP_3竞争性蛋白结合实验。结果:基础状态下妊娠与未妊娠子宫颈平滑肌IP_3水平分别为(82±9)和(96±10)nmol·g^(-1)(protein)。ACh 50μmol·L^(-1)孵育5min时IP_3水平达峰,分别为(109±11)和(122±15)nmol·g^(-1)(protein),但对妊娠与未妊娠时的作用差异不显著。阿托品(Atr)1μmol·L^(-1)抑制ACh促IP_3生成作用。结论:基础状态时的妊娠妇女子宫颈平滑肌细胞IP_3水平高于未妊娠妇女,ACh促进子宫颈平滑肌细胞IP_3合成。

AIM: To detect inositol 1,4,5-triphosphate (IP3) formation of pregnant and nonpregnant human myometrial cells induced by acetylcholine (ACh). METHODS: [3H] IP3 competitive protein binding assay. RESULTS: Basal levels of IP3 in pregnant and nonpregnant human myometrial cells were (82±9) and (96±10) nmol·g-1 (protein), respectively ( n = 6). Incubated with ACh 50 μmol·L-1 for 5 min, IP3 reached the peak levels (109±11) and (122±15) nmol·g-1 ( protein), respectively ( n = 6), but difference of the increments of IP3 in pregnant and nonpregnant women was not significant. The increased IP3 induced by ACh was inhibited by atropine(Atr) 1 μmol·L-1. CONCLUSION: The basal IP3 level in pregnant cervix myometrial cells was higher than that in nonpregnant women. ACh increased the IP3 formation.