光诱导金丝桃素对人鼻咽癌细胞CNE-2的毒作用和凋亡研究

Photocytotoxic and apoptosis of hypericin-mediated on nasopharyngeal carcinoma cell line of human in vitro

目的:探讨以发光二极管为光源,金丝桃素光动力疗法对体外培养鼻咽癌细胞CNE-2的毒作用和凋亡影响。方法:体外培养CNE-2细胞分别加入不同浓度的金丝桃素,另设血卟啉阳性对照组,避光培养6h,分别用黄色和红色发光二极管照射90min,积累能量为5.67J/cm2,培养24h后用MTT法检测金丝桃素和血卟啉在光照条件下对鼻咽癌细胞CNE-2的影响,并用流式细胞仪进行细胞周期分析和凋亡检测。结果:MTT结果显示光活化金丝桃素和血卟啉呈剂量依赖性抑制鼻咽癌细胞的生长,IC50分别为0.049和0.650μg/mL;流式细胞仪分析表明金丝桃素经光照后,可使细胞停留在S期和G2期,并诱导细胞凋亡,0.20μg/mL金丝桃素作用18、28、48h,凋亡率分别达到9.97%、72.19%、92.24%。结论:金丝桃素经光诱导后,可有效抑制体外培养鼻咽癌细胞的增殖,并诱导鼻咽癌细胞凋亡。

AIM： To study effects of hypericin associated with light emitting diode irradiation on nasopharyngeal carcinoma cell line of human in vitro. METHODS： CNE-2 cells were exposed respectively to different concentration of hypericin,and compared with hematoporphyrin as positive control group,and incubated for 6 h in the dark, then accumulative radiated energe of yellow and red light irradiation equivalent to 5. 67 J/cm2 were given throughout 90 min,killing effect and apoptosis of CNE-2 cells were detected by MTT assay after incubation of 24 h and flow cytometry. RESULTS： MTT assay showed that cytotoxicity of hypericin and hematoporphyrin with light irradiation presented in dose-dependent way,their IC50 values were 0. 049 and 0. 650 μg/mL,respectively. Flow cytometry showed that hypericin with light irradiation could block cell growth at S phase and G2 phase,when CNE-2 cells were exposed to hypericin at 0. 20 μg/mL for 18,28 and 48 h,the apoptosis rate reached at 9. 97% ,72. 19% and 92. 24% . CONCLUSION： Hypericin with light irradiation could obviously inhibit the growth of CNE-2 cells and induce apoptosis.