RP-HPLC法同时测定关黄柏中小檗碱、药根碱、巴马汀及黄柏酮含量的方法学研究

Simultaneous determination of jatrorrhizine,palmatine,berberine,and obacunone in Phellodendri Amurensis Cortex by RP-HPLC

目的:建立HPLC法在相同色谱条件下同时测定关黄柏中小檗碱、巴马汀、药根碱和黄柏酮的含量。方法:采用RP-HPLC法梯度洗脱。Phenomenex Gemini C18色谱柱(4.6 mm×250 mm,5μm);检测波长345 nm(检测小檗碱、药根碱、巴马汀)、210 nm(检测黄柏酮);流速l mL.min-1;柱温25℃。结果:在一定范围内,盐酸小檗碱、盐酸巴马汀、盐酸药根碱和黄柏酮峰面积积分值Y与进样量X线性关系良好,加样回收率分别为98.94%,101.17%,96.22%,98.90%,具有较好的精密度、重复性和稳定性。同时对关黄柏药材市场品进行了含量测定。结论:该方法用于测定黄柏药材中小檗碱、巴马汀、药根碱和黄柏酮的含量操作简便、准确、重复性好。

Objective： To develop a HPLC method to determine the content of jatrorrhizine,palmatine,berberine,and obacunone in Phellodendri Amurensis Cortex simultaneously.Mehtod： The separations were carried out at 25 ℃ on a Phenomenex Gemini C18 column（4.6 mm×250 mm,5 μm） eluted with acetonitril and water containing 0.1% phosphoric acid in gradient mode.The flow rate was 1.0 mL·min-1,detection wavelengthes were 345 nm for jatrorrhizine,palmatine,berberine and 210 nm for obacunone.Result： The average recoveries of jatrorrhizine,palmatine,berberine,and obacunone were 98.94%,101.17%,96.22% and 98.90%,respectively.Conclusion： The method is simple,accurate and repeatable,and can be used in content determination of jatrorrhizine,palmatine,berberine,and obacunone in Phellodendri Amurensis Cortex.