厚朴与凹叶厚朴群体遗传学研究

Population genetic study of Magnolia officinalis and Magnolia officinalis var. biloba

目的:对厚朴与凹叶厚朴的群体遗传学进行研究,为中药厚朴的质量控制提供分子生药学方面的依据。方法:对厚朴与凹叶厚朴15个居群应用2个叶绿体基因间序列psbA-trnH和trnL-trnF进行PCR扩增并测序,计算厚朴与凹叶厚朴单倍型频率,用程序HaploNst分析遗传多样性和遗传结构,应用TCS version 1.13软件构建单倍型网状进化树。结果:厚朴与凹叶厚朴均无特有单倍型存在,但单倍型频率存在显著差异,已开始出现遗传分化的趋势,NST略大于GST。结论:厚朴与凹叶厚朴在遗传上已出现遗传分化的趋势,但尚未完全分化成彼此独立的单系。

Objective： population genetic study of Magnolia officinalis and M.officinalis var.biloba was performed in this paper.The objectives of this study are to provide baseline data of molecular pharmaceutics for quality control of Cortex Magnoliae Officinalis.Method： A total of 15 populations of M.officinalis and M.officinalis var.biloba were collected.PCR amplification and sequencing were performed with two chloroplast intergenic spacers psbA-trnH and trnL-trnF.Chloroplast haplotype frequencies were calculated,genetic diversity and genetic structure were estimated by using the program HAPLONST,and a haplotype network depicting the mutational relationships among distinct haplotypes was drawn following the principle of parsimony by TCS version 1.13.Result： The differentiation of haplotype frequencies between M.officinalis and M.officinalis var.biloba was significant although there was no private haplotypes of themselves.Conclusion： There was certain genetic divergence between M.officinalis and M.officinalis var.biloba although the monophyletic clade of themselves was not still formed.