甘草酸二铵-壳聚糖纳米粒制备与药效学评价

Preparation and pharmacodynamic evaluation of diammonium glycyrrhizinate-loaded chitosan nanoparticles

目的：制备甘草酸二铵-壳聚糖纳米粒（DG-CS NPs）,评价其制剂学性质及对溃疡性结肠炎的治疗效果。方法：喷雾干燥法制备DG-CS NPs,正交设计法优化;考察纳米粒的形态、粒径分布及体外释放特征。硫酸葡聚糖诱导溃疡性结肠炎（UC）模型小鼠,以疾病活动指数法评价DG-CS NPs的治疗效果。结果：DG-CS NPs大小在300-600 nm,载药量为（51.25±1.75）%。DG-CS NPs的药物释放受环境pH的影响,对UC具有显著的治疗与改善效果。结论：喷雾干燥法制得DG-CS NPs对UC模型小鼠具有治疗作用。

Objective： To prepare the diammonium glycyrrhizinate-loaded chitosan nanoparticles（DG-CS NPs）,and evaluate its pharmaceutical properties and pharmacodynamic effects on ulcerative colitis（UC）.Method： DG-CS NPs were prepared by spray drying method and optimized by orthogonal design.The morphology,size and in vitro release of DG-CS NPs were investigated.The therapeutic effects of DG-CS NPs on UC mice induced by dextran sulfate were evaluated preliminarily through disease active index method.Result： The size of DG-CS NPs with loading capacity about（51.25±1.75）% was in the range of 300-600 nm.The release of DG-CS NPs was associated with environmental pH value and displayed significant therapeutic and preventive effects on UC.Conclusion： DG-CS NPs prepared by spray drying method showed efficacy on UC mice.