青风藤提取物聚乳酸缓释微球的制备及体外释药性能的研究

Preparation of sustained release microspheres containing extracts from Caulis sinomenii with polylactic acid and their release property in vitro

目的:制备含青风藤总生物碱提取物的聚乳酸(PLA)微球,并考察其体外释药性能。方法:采用乳化-溶剂挥发法制备青风藤总碱PLA微球,对其形态、包封率、粒径分布进行研究,并探讨了制备条件对PLA微球释药性能的影响。结果:青风藤总碱PLA微球外观圆整,粒径分布均匀,平均包封率和载药量分别为(83.4±5.63)%,(8.7±0.35)%,平均粒径为(21.5±1.22)μm,32 h体外累积释药量为80%。结论:本研究成功制备了青风藤总碱PLA微球;通过控制不同因素,可得到具有良好缓释性能的青风藤总碱PLA微球。

Objective： To prepare polylactic acid microspheres containing total alkaloid extracts of Caulis sinomenii and study their release characteristics in vitro.Method： Polylactic acid microspheres containing total alkaloid extracts of C.sinomenii were prepared by O/W emulsification solvent-evaporation process.The microspheres were characterized in terms of morphology,encapsulation efficiency,and particle size distribution.The effect of different conditions on release property of microspheres was studied.Result： The formed microspheres were spherical with smooth surfaces.The encapsulation efficiency and rate of drug loading were（83.4±5.63）% and（8.7±0.35）%,respectively.The distribution of particle size was uniform and the average size was（21.5±1.22） μm.In vitro release study revealed that the 32-hour accumulative release percentage reached 80%.Conclusion：Polylactic acid microspheres containing total alkaloid extracts of C.sinomenii were prepared successfully.Microspheres with good sustained-release characteristics can be produced by controlling different process parameters.