均匀设计法优选桂枝茯苓丸方药的半仿生提取工艺条件

Optimization of extraction conditions for semi-bionic extraction of Guizhi Fuling pill decoction by uniform design

目的:优选桂枝茯苓丸方药的半仿生提取工艺条件。方法:将方中含挥发性成分中药超临界萃取后的药渣与方中茯苓混合,以芍药苷、肉桂酸、苦杏仁苷、茯苓酸、总多糖、干浸膏为指标,采用均匀设计优选其半仿生提取法(简称SBE法)的工艺条件。结果:优化条件为3煎用水pH依次为4.98,7.35,8.00,煎煮总时间为4.99 h。结论:结合生产实际,确定其SBE法工艺条件为3煎用水的pH依次为5.0,7.5,8.0;煎煮时间依次为2.0,1.5,1.5 h。

Objective： To optimize the extraction conditions for semi-bionic extraction of Guizhi Fuling pill decoction.Method： The best extraction condition of the semi-bionic extraction for Guizhi Fuling pill decoction was optimized by uniform design was optimized with paeoniflorin,cinnamic acid,laetrile,pachymic acid,total polysaccharose and dry extract as the indexes.Result： The optimized extraction condition is that extracting for 3 times with solvent pH values of 4.98,7.35 and 8.00,respectively,and the total extracting time was 4.99 h.Conclusion： In consideration of manufacturing fact,the pH value of thrice extractions is set at 5.0,7.5,8.0 respectively,and the extracting time is 2.0,1.5,1.5 h in turn.