小麦持久抗病品种N.Strampelli的抗条锈病基因分析和微卫星标记

Genetic analysis and molecular mapping of stripe rust resistance gene in durable wheat variety N. Strampelli

N.Strampelli是由意大利引入我国的小麦持久抗病性品种,对我国目前多数的条锈菌流行小种均有良好的抗性。为了明确其抗条锈病基因的遗传机制,利用小麦条锈病小种CYR30、CYR31、Su-4和Su-14对N.Strampelli与中国春杂交后代进行遗传分析,结果表明N.Strampelli对CYR30、CYR31的抗病性均由1对显性基因和1对隐性基因互补控制,对Su-14、Su-4的抗病性各由1对隐性基因控制,将其中控制Su-14抗病性的隐性基因暂时命名为YrNS-1。利用分离群体分析法(BSA)对接种Su-14的正交F2代群体进行SSR分子标记,在1BL上找到4个与YrNS-1紧密连锁的微卫星标记Xwmc719、Xgwm124、Xwmc44和Xcfa2147,遗传距离分别为3.2、4.6、5.7和10.3cM。与已知位于1BL染色体上的抗条锈基因比较分析表明,YrNS-1可能是1个新的抗条锈病基因。

N.Strampelli,a durable resistant wheat variety imported from Italy,is resistant to the currently prevailing races of Puccinia striiformis f.sp.tritici in China.To determine genetics of the resistance gene,the progeny population of N.Strampelli /Chinese spring,were tested with prevalent race CYR30,CYR31,Su-4 and Su-14 at seedlings stage.The results indicated that one dominant gene and one recessive gene complementarily conferred to CYR30 and CYR31,resistance to the physiological race Su-4 and Su-14 were controlled by one recessive gene,respectively,which conferred to Su-14 temporarily designated as YrNS-1.Using BSA（bulked segregate analysis）methods and SSR（simple sequence repeats）marker polymorphic analysis to map the gene in F2 population,four SSR markers Xwmc719,Xgwm124,Xwmc44 and Xcfa2147 on the chromosome arm 1BL were found to be linked to YrNS-1.The genetic distances for these markers were 3.2,4.6,5.7 and 10.3 cM,respectively.The comparison with the other stripe rust resistance genes located on 1BL suggested that YrNS-1 might be a novel gene.