高压微射流制备纳米中链脂肪酸脂质体的研究

Preparation of Medium-Chain Fatty Acids (MCFA) Nano-Liposome by Means of High Pressure Microfluidization(HPM)

以中链脂肪酸(MCFA)为模型材料,比较了高压微射流(HPM)、超声波和微孔过滤3种方法处理后的MCFA脂质体,着重研究了HPM的处理压力和处理次数对脂质体的平均粒度、包封率和稳定性的影响。结果表明:相比超声波和微孔过滤法,HPM处理后的MCFA脂质体的平均粒度最小、包封率最高、稳定性良好;在120 MPa压力条件下处理6次时,MCFA脂质体的平均粒度达到最小,为(73.9±10.2)nm,包封率为(52.20±9.57)%;在120 MPa压力条件下处理4次时,包封率和载药量达到最高,分别为(70.64±11.25)%和(9.42±0.83)%,稳定性系数最高,为0.9990±3.6951,平均粒度为(78.9±21.5)nm。

Medium-chain fatty acids（MCFA） nano-liposome was prepared and characterized by means of high pressure microfluidization（HPM）,sonication and microfiltration,respectively.The average diameter,encapsulated efficiency and stability were investigated after treatment with different pressures and passes number.The results indicated that MCFA liposome prepared by HPM exhibited the smallest average diameter,the largest encapsulated efficiency and the best stability.The smallest average diameter of MCFA liposome could be achieved after a treatment of 6 passes at 120 MPa,with a value of（73.9±10.2） nm,while the encapsulated efficiency was（52.20±9.57）%.The largest encapsulated efficiency of（70.64±11.25）% and loaded efficiency of（9.42±0.83）% were obtained when treated 4 passes at 120 MPa,with an average diameter value of（78.9±21.5） nm.After being treated 4 passes,MCFA liposome exhibited the best stability,with a coefficient value of 0.999 0±3.695 1.