MPTP对鼠肝线粒体和微粒体谷胱甘肽含量的影响

EFFECT OF 1-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE ON LEVELS OF GLUTATHIONE IN HEPATIC MITOCHONDRIA AND MICROSOME OF MICE

本文报道给雄性C_(57)小黑鼠腹腔注射两种剂量MPTP后,发现组2(MPTP35mg/kg每日1次7天)鼠肝线粒体和微粒体的谷胱甘肽(GSH)含量降低,比对照缉分别降低280.1％和32.4％(P<0.01)。组3(MPTP35mg/kg,4小时1次,共3次)鼠肝线粒体的GSH含量仅轻度下降,比对照组下降21.3％(P<0.05),微粒体的GSH含量则无改变。结果提示MPTP有降低鼠肝GSH含量的作用,并与其剂量有关。作用机理推测是由于GSH存清除MPTP诱致体内产生过量自由基的过程中自身过度耗竭,或是由于MPTP诱致GSH的合成障碍。

Male C57 black mice showed moderately decreased hepatic glutathione (GSH) corteat 72 h after the injection of 1-methy1-4-pheny-1,2,3,6-tetrahydropyridine (MPTP), 35 mg/kg once a day for 7 days. In comparison with control, the levels of GSH in the hepatic mitochondria and microsomes were reduced by 28.1% and 32.4% respectively (p<0.01). Another group of mice received 3 injections of 35mg/kg at 4 intervals in a single day, exhibited slightly reduced GSH concentrations in the hepatic mitochondria, which were depleted by 21.3% compared with control (p<0.05). whereas GSH levels of hepatic microsomes were not affected. The result indicates that MPTP has dose-related effect of decreasing the hepatic GSH content of mice. It is suggested that our findings of low hepatic GSH concentrations in MPTP-treated mice may be due to excessive consumption of GSH in detoxification processes, or to MPTP-induced deficiency of GSH synthesis.