大豆贮藏蛋白11S和7S组分及其亚基相对含量的研究

Content Variation of 11S,7S Globulins and Their Subunits of Soybean Storage Protein

选取晋大62与高蛋白品种诱处4号杂交亲本及后代的40个品系种子样品为材料,利用聚丙烯酰胺凝胶电泳技术(SDS-PAGE),得到贮藏蛋白的各个条带组分图,通过分析大豆贮藏蛋白11S和7S组分及其亚基的相对含量,来探索大豆贮藏蛋白11S和7S组分的关系及在育种上的利用。SDS-PAGE电泳图谱显示:亲本及杂交后代群体贮藏蛋白亚基的组成基本相同,没有出现亚基缺失现象,但是不同品系间各亚基存在显著变异。研究结果为今后提高大豆蛋白品质,选育优质大豆新品种提供了理论基础和实践依据。

Soybean Jinda 62,high protein Youchu 4,and 40 lines of their hybrid descendants were selected as test materials,the band pictures of the fractions of their storage proteins were got with SDS-PAGE electrophoresis.The relationship between 11S and 7S globulins of soybean storage protein and its application in breeding was explored through analyzing the content variations of the 11S,7S globulins and their subunits.Results：The subunit compositions of soybean storage protein for parents and hybrid progenies are basically the same,but there is significant variation in the subunit content for different line.The results provide a basis to improve soybean protein quality and breed high quality variety.