中国对虾淋巴组织培养中的病毒及病理观察

VIRAL AND PATHOLOGICAL OBSERVATION IN CULTURED LYMPHOID TISSUES OF SHRIMP PENAEUS CHINENSIS

通过对中国对虾的淋巴器官和经过培养的养殖、海捕中国对虾的淋巴组织细胞进行电镜观察 ,均发现在细胞内存在一种形状为球形 ,有囊膜 ,平均直径为 136nm的病毒 ,病毒分布于细胞质内 ,或成团存在 ,似为一种虹彩病毒 ;在养殖对虾淋巴器官及培养过的组织细胞中还发现另一种病毒 ,病毒粒子为正二十面体 ,无囊膜 ,平均直径为 33nm ,分布于细胞质中 ,似为一种小RNA病毒。在体外培养淋巴组织过程中 ,病毒在细胞内没有明显增殖迹象。感染病毒的细胞呈现细胞核固缩 ,细胞质空泡化 ,线粒体内嵴模糊 ,粗面内质网水肿等一系列病理变化。对淋巴组织切片进行光镜观察 ,发现淋巴器官组织部分坏死 ,有些细胞核肿大 ,苏木精深染 ,严重的细胞核结构已经被破坏。

The lymphatic organ of shrimp Penaeus chinensis and the cultured lymphatic tissues from farmed and wild shrimps were examined under the transmission electronic microscope (TEM). The particles with envelope, which are icosahedron in shape and 136 nm in diameter, were found in the cytoplasm in all samples. It seemed to be the Iridovirus. Another virus was found in the cytoplasm of lymphatic tissue of farmed shrimp. The particles were icosahedron, 33 nm in diameter and without envelope, which seemed to be the Picornavirus. No significant replication of the viruses was found in the cultured cells. Pathological changes , including karyorrhexis, nuclear hypertrophy, cytoplasm vacuolization, tissues necrosis of lymphatic organ were also observed.