稻瘟病菌有性世代及色素的基因表达分析

EXPRESSION OF GENES CONTROLLING PERFECT STATE AND MELANIN IN MAGNAPORTHE GRISEA

利用分离自陆稻的稻瘟病菌菌株94-84c(MAT1-1)和95-23-4a(MAT1-2)进行有性杂交,得杂交组合6315;利用本实验室子囊孢子菌株6315R-11(MAT1-1)与中国水稻所提供的6023菌株(MAT1-2)杂交得组合7368;6315R-31(MAT1-1)与95-23-4a(MAT1-2)杂交得组合7370;陆稻分离菌94-80d(MAT1-1)与95-23-4a(MAT1-2)杂交得组合6307。通过6315、7368、7370等3个杂交组合有性世代形成的遗传表现和6307组合色素产生的遗传表现分别对有性世代和色素进行初步的基因表达分析。结果显示,稻瘟病菌的有性世代受2个基因控制,分别定名为PS1基因和PS2基因;色素则仅由一个基因控制,定名为M基因。对有性世代及色素遗传进行深入研究,将为快速、有效地培育抗瘟品种及指导抗侵入杀真菌剂的研制提供理论依据。

Cross 6315 was made by intercrossing conidial isolate 94-84c (MAT1-1) and 95-23-4a(MATl-2) , both isolates were derived from upland rice. Cross 7368 was made by 6315R-1KMATM) and 6023CMAT1-2), cross 7370 by 6315R-31 (MAT1-1) and 95-23-4a(MATl-2), cross 6307 by 94-80d (MATl-1) and 95-23-4a (MAT1-2) in the same way. The genetic expression of perfect state was studied by analyzing cross-fertility of the progenies from cross 6315, cross 7368 and cross 7370. Meanwhile, genetic expression of melanin was studied by observing melanin formation of the progenies from cross 6307. Results indicated that perfect state was controlled by two genes, named as PS1 and PS2,but melanin only controlled by one gene, named as M. These results would provide theoretical instruction for rice breeding resistance to blast disease and developing anti-penetrant fungicides.