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酵母双杂交技术筛选朊蛋白PrP23-231互作蛋白 被引量：5

Screening Proteins Interacting with PrP23-231 Using Yeast Two-hybrid System
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摘要 利用酵母双杂交技术,以pSos-PrP23-231载体为诱饵质粒,筛选小鼠脑cDNA文库中能与朊蛋白PrP23-231相互作用的蛋白。通过一次筛选,三次验证,从小鼠脑cDNA文库中得到2个阳性克隆,通过BLAST序列比对得到2个候选基因序列,并对验证后的阳性克隆的外源性片段进行测序及同源性分析,得到的蛋白编码基因可能与朊蛋白存在相互作用,其作用可能与疯牛病发病机制有关。 The mouse leukocyte cDNA library was screened with pSos-PrP23-231 as bait plasmid by yeast two hybrid system,then the positive yeast clones were obtained. The candidate gene were confirmed by DNA sequencing and BLAST analysis in NCBI,the gene sequence were obtained with their coding protein interacting with pSos-PrP23-231,this candidate genes possibly involve with pathogenesis of BSE.
作者 宋博翠 陈志宝 万家余 高宏伟
机构地区 黑龙江八一农垦大学 军事医学科学院军事兽医研究所
出处 《中国畜牧兽医》 CAS 北大核心 2010年第8期34-37,共4页 China Animal Husbandry & Veterinary Medicine
关键词 朊蛋白 酵母双杂交 蛋白互作 prion yeast two-hybrid system protein interaction
分类号 Q51 [生物学—生物化学]
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参考文献9

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二级参考文献46

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共引文献13

同被引文献55

  • 1吴娟,钱凯,杨泽峰.酵母双杂交系统的研究进展[J].安庆师范学院学报（自然科学版）,2005,11(2):59-63. 被引量：7
  • 2张佩景,李慧艳,潘欣,靳宝锋,满江红,李卫华,张学敏,张涌.酵母双杂交技术研究与人孕酮受体B相互作用的蛋白质[J].生物技术通讯,2005,16(6):612-614. 被引量：4
  • 3张莉,黄倢.酵母双杂交系统的改进与研究进展[J].海洋湖沼通报,2006(1):108-116. 被引量：5
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引证文献5

二级引证文献18

中国畜牧兽医
2010年 第8期

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