鹅源鸭疫里默氏杆菌的分离和鉴定

Isolation and Identification of Riemenella anatipestifer from Geese

为研究鹅源鸭疫里默氏杆菌的生化特性、药敏情况、血清型和致病性,从扬州郊区发病鹅场分离到1株细菌,经分离培养、染色镜检、生化特性鉴定、玻片凝集试验,确定为Ⅱ型鸭疫里默氏杆菌。对病料进行病毒分离试验,经过血凝试验和琼脂扩散试验,没有发现鹅新城疫病毒、禽流感病毒和小鹅瘟病毒。动物致病性试验结果表明,鸭疫里默氏杆菌分离菌株可以通过静脉注射、皮下注射和滴鼻3种途径感染鹅,出现100%的死亡率,说明分离株对扬州鹅具有很强的致病性,同时提示在鹅的免疫计划中也应该充分考虑到鸭疫里默氏杆菌的致病和传播。

The purpose of this paper was to study biochemical characteristics, drug sensitivity, serotype and pathogenic infection of Riemerella anatipestifer（RA） from geese. One goose isolate from an infected farm of the suburb of Yangzhou were isolated, cultured, stained, tested by microscopy, biochemical identified, and slide agglutination test. The results showed that the isolate was Ⅱ serotype RA. While no Newcastle disease virus, avian influenza virus, and gosling plague virus were isolated by hemagglutination test （HA） and agar-gel precipitin test （AGP）. At the same time, the isolate was used to infect 15-days-old geese,the isolate attacked the geese through intravenous injection, subcutaneous injection and intranasal three infection ways. The results showed that RA had strong pathogenicity to Yangzhou geese, which prompted us, the Rimer ella anatipestifer pathogenicity and dissemination should also be taken fully into account in the immunization programs for geese.