油菜基因转移适宜苗态建成方法研究

Study on the Method of Forming Optimum PlantShape for Rape Gene Transformation

油菜种子经２～５℃低温处理及冬、春两季１０个播期试验，研究了采用真空渗入法对油菜进行ＣａＭＶＣＡＢＢ－ＢＪＩ和Ｂａｒｉ－Ⅰ株系基因Ⅵ转移时适宜植株形态的建成方法。结果表明，芥菜型油菜低温处理１０，２０，３０ｄ，均可明显促进抽薹，春季３月１８日播种或冬季１０月１１日播种最适于形成理想的转化态植株。甘蓝型及白菜型油菜种子经低温处理不能通过春化。甘蓝型油菜冬季１０月２６日播种，白菜型油菜冬季１０月１１日播种或春季３月１２日播种，易形成适于转移的植株形态。

By treating the seeds of turnip rape (B.campestris),brown mustard (B.juncea) and rape (B.napus) at 2-5 low temperature for 10 days,20 days,30 days,40 days,0 days and sowing on ten different dates in winter and spring,the ways to form optimum plant shape are studied with vaccum infiltration method transforming the CaMV CABB BJI gene into the rape.The results indicate that treating the seeds of brown mustard at low temperature for 10 days can obviously promote plant extending bolts.Seed sowing on 18 Mar.in Spring and 11 Oct.in winter is best to form optimum plant shape of gene transformation.The treatment of rape and turnip seeds at low temperature shows no effect on their vernalization.Rape sowing on 26 Oct.and turnip rape sowing on 11 Oct.in winter or 12 Mar. in spring are respectively the most suitable ways to form optimum plant shape of gene transformation.