中国李原生质体培养及植株再生

Protoplast Culture and Plant Regeneration of Chinese Plum

对中国李（ＰｒｕｎｕｓｓａｌｉｃｉｎａＬｉｎｄｌ．）原生质体分离和培养的有关因素进行了研究。以悬浮培养物为材料，在适宜的酶解液中酶解１２ｈ，原生质体产量和活力分别达到３×１０７个／ｇ和９５％．以改良ＭＳ为基本培养基，在培养初期加２，４－Ｄ１．０ｍｇ／Ｌ，ＢＡ０．５ｍｇ／Ｌ，培养３０ｄ后将ＢＡ调至１．０ｍｇ／Ｌ，以０．５５ｍｏｌ／Ｌ葡萄糖调节渗透压，在２×１０５个／Ｌ的植板密度下液体浅层培养５０ｄ后形成了微愈伤组织。微愈伤组织转至固体培养基上继代培养，在分化培养基上分化出不定芽，在生根培养基上生根形成完整植株。

Some conditions of protoplast isolation and culture of Chinese plum (Prunus salicina Lindl.) were studied.The protoplast yield and viability of suspension cultures could amount to 3107 protoplasts/g and 95%,respectively,when incubated in suitable enzyme solution for 12 hours.Modified MS medium,with 1.0 mg/L 2,4D and 0.5 mg/L BA during beginning stage and BA concentration adjusted to 1.0 mg/L after 30 days culture,was a suitable culture medium.Osmoticum of the medium was adjusted to 0.55 mol/L with glucose.Microcalli were developed after 50 dayss culture in a liquid layer with plating density of 2105/L.The microcalli were subcultured on solid medium and developed adventitious buds on diffentiation medium.Eventually,shoots rooted and developed into whole plantlets on rooting medium.