五种血清型柯萨奇病毒的实时荧光RT-PCR检测方法研究

Research on Detection of Five Serotypes of Coxsackie Viruses with Real-Time RT-PCR

在柯萨奇病毒基因组VP1区设计引物和探针,分别建立了特异性检测5种血清型(A9、A16、B2、B3和B5型)柯萨奇病毒(Coxsackievirus)基于MGB探针的实时荧光RT-PCR方法。通过构建的分别适于5种血清型病毒检测的质粒标准分子对建立的检测体系进行了灵敏度分析,确定5种血清型柯萨奇病毒检测体系的检测下限均为2拷贝质粒标准分子DNA。

Based on the VP1 region of Coxsackivirus genome, primer and probe sets have been designed to detect five serotypes of Coxsackieviruses （A9, A16, B2, B3 and B5） separately by real - time RT - PCR. Plasmid standard molecules suitable for coxsaekieviruse A9, A16, B2, B3 and B5 detection were constructed respectively. Limits of detection （LODs） of the developed detection systems for five serotypes of Coxsackieviruse were all 2 copies of plasmid standard molecule DNA.