磺胺嘧啶在欧洲鳗鲡体内的药代动力学研究

Pharmacokinetics of Sulfadiazine in European eels (Anguilla anguilla)

在(25±1)℃水温条件下,以300 mg/kg的单剂量,给欧洲鳗鲡混饲口灌磺胺嘧啶后,用高效液相色谱法测定血浆和肌肉中的药物浓度,研究了磺胺嘧啶在欧洲鳗鲡体内的药代动力学特征。结果表明:欧洲鳗鲡血浆和肌肉中磺胺嘧啶经时过程均符合一级吸收二室开放模型,其理论方程为:C血浆=196.16e-0.0308 t+44.028e-0.0067 t-7.9584e-0.0871 t,血浆中药物主要动力学参数如下:T1/2ka为7.9584h;T1/2a为22.473h;T1/2β为104.27h;Tmax为21.512h;Cmax为100.51mg/L。C肌肉=0.077e-0.061 t+34.545e-0.015 t-3.8399e-0.181 t,肌肉中主要动力学参数如下:T1/2ka为3.8399h;T1/2a为11.277h;T1/2β为46.210h;Tmax为15.762h;Cmax为25.024mg/kg。以20μg/kg为最高残留限量计算,在本试验条件下,建议磺胺嘧啶在欧洲鳗鲡体内的休药期不低于48d。

In order to study the pharmacokinetics of Sulfadiazine （ SD ） in European eels （ Anguilla anguilla ）, the eels were treated orally with Sulfadiazine - blended feeds at a single dose of 300mg· kg^ - 1 body weight at water tem- perature of （25± 1 ） ℃. The SD concentrations of plasma and muscle were determined by high performance liquid chromatograph）; （HPLC）. The results showed that both the plasma and muscle concentration - time course of SD conformed to a two - compartme -nt open model with the first order absorption. The pharmacokinetic equations were as follows： Cplasma = 196.16e^-0.0308t ＋ 44.028 e^-0.0067t - 7. 9584e^-0.0871t, the main pharmacokinetic parameters were：Tt/2ka was 7. 9584h; T1/2a, 22. 473h; T1/2β, 104. 27h; Tmax, 21. 512h; C max,100. 51mg· L^-1. Cmuscle = 0. 077e^-0.061t ＋ 34. 545e ^-0.015t - 3. 8399e^-0.181t, the main pharmaeokinetic parameters were ： T1/2ka was 3. 8399h ; T1/2a, 11. 277h ; T1/2β, 46. 210h ; Tmax, 15. 762h and Cmax was 25. 024mg · kg^-1. According to the maximum residual limit（MRL） of 20μg · kg^-1, the withdrawl period should not be less than 48 days under this experiment condition.