摘要
目的:探讨肠结核患者在胸腺肽-alpha1治疗前后外周血中T细胞亚群、NK细胞的变化与TNF-α基因多态性的相关性。方法:66例肠结核患者在常规抗结核治疗基础上加用胸腺肽-alpha1治疗,采用流式细胞术检测治疗前后外周血T细胞亚群及NK细胞的水平。提取肠结核患者外周血基因组DNA,用PCR-RFLP方法检测个体中TNF-α-238、-308两个多态性位点的基因型,并采用SPSS软件进行多态性与治疗前后T淋巴细胞亚群变化的相关性分析。结果:66例患者在常规抗结核治疗基础上加用胸腺肽-alpha1治疗后,统计结果显示在治疗第16周结束时,TNF-α-308GA+AA基因型组合的患者中CD3+、CD4+、CD8+、CD4+/CD8+、NKcells检测值均较治疗前明显增高,差异均具有显著的统计学意义(P<0.05);而TNF-α-308GG基因型以及TNF-α-238多态性位点免疫学检测值均未显示出统计学差异(P>0.05)。结论:TNF-α基因启动子区多态性与肠结核患者胸腺肽-alpha1治疗前后T淋巴细胞亚群变化有着明显的关联。
OBJECTIVE：To investigate the association between the polymorphisms of TNF-α gene and the changes of T cell subsets and NK cells before and after the treatment of thymosin-alpha 1 in the intestinal tuberculosis （TB） patients.METHODS：66 cases of intestinal tuberculosis patients were treated with thymosin-alpha 1 plus the conventional anti-TB treatment.The levels of peripheral blood T cell subsets and NK cells were determined by flow cytometry.DNA was extracted from patients＇peripheral blood and the genotypes of TNF-α-238,-308 polymorphisms were detected by PCR-RFLP.The correlation analysis between the polymorphisms and T cell subsets was performed by using SPSS software.RESULTS：After treatment with the conventional anti-TB therapy combined with thymosin-alpha 1 therapy,the statistical results at the end of 16 weeks showed that the detected values of CD3 ＋ ,CD4 ＋ ,CD8 ＋ ,CD4 ＋/CD8 ＋ ,and NK cells in patients with TNF-α-308 GA ＋ AA genotype combination were significantly higher than before treatment,and the differences were statistically significant （P0.05）; however,the detection on TNF-α-308 GG genotype and TNF-α-238 polymorphism showed no significant difference compared with before treatment （P0.05）.CONCLUSION：There is a significant correlation between the polymorphisms of TNF-α gene and the alterations of T cell subsets in the intestinal tuberculosis patients treated with thymosin-alpha 1.
出处
《中国医院用药评价与分析》
2010年第8期721-723,共3页
Evaluation and Analysis of Drug-use in Hospitals of China
