新生儿轮状病毒抗体水平及轮状病毒排出情况调查

INVESTIGATION ON ROTAVIRUS ANTIBODY LEVEL AND EXCRETION OF ROTAVIRUS IN NEWBORN

1988年10月7日至11月17日,我们采集了31名健康剖腹产新生儿的脐带血及其出生后1至7日的155份粪标本。应用ELISA法检测血标本中的轮状病毒(RV)抗体,结果,11份(35.48％)具有高滴度RV抗体(≥1:800),14份(45.16％)具有中等滴度RV抗体(1:200～1:400),仅6份(19.35％)RV抗体滴度处于低水平(1:100),RV抗体几何平均滴度(GMT)为319.86±4.48粪标本经ELISA检测未发现RV抗原、经免疫电镜法(IEM)观察也未发现病毒颗粒。说明了这些剖腹产新生儿未感染RV,并从他们的母亲那里获得了RV抗体。

Thirty-one serum samples were collected from the blood vessel of umbilical cord of healthy newborn babies who were delivered by caesarean section and 155 fecal samples were collected from them at 1-7 days after birth. Titer of antibody to rotavirus was assessed by ELISA. Eleven (35.48%) samples contained high titer antibody (≥1:800),14 (45.16%) were at medium level (1:200-1:400), and only 6 (19.35%) at low level (1:100). Geometric mean titer of all samples was 319.86±4.48. There was no antibody and virus granules in all the feces specimens. It showed that the neonates by caesarean birth were not infected with rotavirus and the antibody to rotavirus was acquired from their mothers.