MiR-141增强人血管内皮细胞增殖及迁移能力

MiR-141 increases proliferation and migration of human vascular endothelial cells

目的研究microRNA对血管内皮细胞增殖及迁移能力的影响,以阐明microRNA在血管新生过程中发挥作用的分子机制。方法用microRNA的反义寡核苷酸序列封闭microRNA的功能后,用四甲基氮唑蓝微量酶反应比色法(MTT)进行筛查,分析血管内皮细胞增殖活性。随后封闭或过表达这些microRNA,用MTT、集落形成和迁移实验检测细胞的增殖和迁移能力。结果有7种microRNAs对血管内皮细胞的生长有促进作用。其中封闭miR-141的功能后,细胞生长受抑制最明显,且集落形成能力和细胞迁移能力也明显下降。相反,过表达miR-141后,细胞增殖和迁移能力明显增强。结论 miR-141能够增强血管内皮细胞的增殖和迁移能力,起到促进血管新生的作用。

Objective In this study,the effects of microRNA on proliferation and migration of endothelial cells were examined to illuminate the molecular mechanisms of microRNA in angiogenesis.Methods MicroRNA was blocked in endothelial cells and the changes of cell proliferation were detected with methyl thiazolyl tetrazolium（MTT） assay.Subsequently,microRNA was blocked or overexpressed in endothelial cells and the changes of cell phenotypes were detected with MTT assay,colony-forming test and migration assay.Results Consequence of MTT assay demonstrated that 7 microRNAs promoted growth of endothelial cells.With suppression of miR-141,the endothelial cells proliferation,migration and the colony formation were all significantly inhibited.However,the change of cell phenotypes was reversed when miR-141 overexpressed.Conclusion miR-141 promotes proliferation and migration of endothelial cells and increases angiogenesis.