临床宫颈组织标本HPV16 L1蛋白检测

The detection of HPV16 L1 from cervical tissue specimen

目的检测HPV16阳性宫颈标本L1蛋白的表达,研究宫颈损伤程度与L1蛋白表达的规律。方法应用PCR法对宫颈炎、宫颈上皮内瘤变(CINⅠ～Ⅱ)、原位癌、早期浸润癌及中晚期癌标本进行型别检测,ELISA法及免疫组化法检测HPV16阳性标本中HPV L1蛋白表达。结果 54例宫颈组织中HPV16阳性为46例(85.2%)。随着宫颈损伤加重,HPV16阳性标本的抗原抗体反应逐渐减弱;免疫组化阳性反应仅出现于上皮组织内,反应强度及分布与宫颈损伤程度及癌变组织的分化状态有关。结论 HPV16 L1蛋白的表达同时受宫颈组织损伤程度及宿主细胞分化状态的影响。早期对HPV L1蛋白进行检测,可为宫颈癌的早期预防及临床诊治提供理论指导。

Objective Detecting the expression of L1 in HPV16 infected cervical tissues and explore the correlation of HPV16 L1 with cervix injured degree.Methods Multiplex PCR was used to detect HPV16-positive specimen in cervicitis,CINⅠ～Ⅱ,cancer in situ and cervical cancer.The expression of L1 was detected in each group by ELISA and immunohistochemistry.Results Forty-six of 54 cases were HPV16 positive（85.2%）.The reaction between antigen and antibody get less significant as the aggravation of cervical damage;the positive reaction appeared in the epithelium tissue in immunohistochemistry,which was effected by tissue＇s damaged degree and differentiated states.Conclusion Higher rate of HPV16 detection in women.L1 expressed in epithelium which affected by tissue＇s differentiation.The expression of HPV16 L1 was less significant with as the damage aggravation.Detecting HPV L1 earlier in clinic can provide the direction for cervical cancer.