摘要
目的:探讨13-甲基十四烷酸(13-MTD)对过氧化氢(H2O2)诱导SH-SY5Y神经细胞损伤的影响。方法:采用体外细胞培养的方法,建立SH-SY5Y神经细胞H2O2损伤模型。光镜观察细胞形态,SRB法检测细胞存活率,AO/EB双重染色法检测细胞凋亡,MTT法检测线粒体活性。结果:与正常对照组相比,H2O2损伤模型组细胞出现明显病理改变,细胞存活率、线粒体活性均显著下降(P<0.01),细胞凋亡率显著升高(P<0.01);13-MTD10、20、40μg/mL可显著提高细胞存活率(P<0.01);13-MTD5、10、20μg/mL可显著下调细胞凋亡率(P<0.01);13-MTD5、10、20、40μg/mL可显著提高细胞MTT代谢率(P<0.01);且存在一定量效差异(P<0.05,P<0.01)。结论:不同剂量的13-MTD对H2O2诱导的SH-SY5Y神经细胞氧化损伤具有保护作用。
AIM:To investigate the protective effects of 13-methyltetradecanoic acid(13-MTD) on the injury of cultured SH-SY5Y neural cells induced by hydrogen peroxide(H2O2).METH-ODS:The injury of cultured SH-SY5Y cells was made in vitro by H2O2.Microscope was used to detect the morphology of cells.Sulforhodamine B assay was applyed to detect the cell viability.Acridine orange/ethidium bromide (AO/EB) double staining was adopted to observe the apoptotic morphology.MTT assay was employed to detect the mitochondrial reducibility.RESULTS:The cells apoptosis rate was increased,the cells viability and mitochondrial reducibility were decreased significantly in group model of H2O2 injury compared with the group control (P〈0.01).13-MTD 10,20,40 μg/mL increased cell survival and 13-MTD 5,10,20 μg/mL reduced the apoptosis rate as well as 13-MTD 5,10,20,40 μg/mL increased the cell MTT metabolic rate compared with the group model of H2O2 injury(P〈0.01),there are some dose-dependent relationship (P〈0.05,P〈0.01).CONCLUSION:Different doses of 13-MTD shows the neuroprotection effects and can protect SH-SY5Y cells from the injury induced by H2O2.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2010年第6期622-626,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
福建省科技计划项目(2010Y0027)