13-甲基十四烷酸对H_2O_2诱导SH-SY5Y神经细胞损伤的保护作用

Protective effects of 13-MTD on injury of SH-SY5Y neuroblastoma cells induced by hydrogen peroxide

目的:探讨13-甲基十四烷酸(13-MTD)对过氧化氢(H2O2)诱导SH-SY5Y神经细胞损伤的影响。方法:采用体外细胞培养的方法,建立SH-SY5Y神经细胞H2O2损伤模型。光镜观察细胞形态,SRB法检测细胞存活率,AO/EB双重染色法检测细胞凋亡,MTT法检测线粒体活性。结果:与正常对照组相比,H2O2损伤模型组细胞出现明显病理改变,细胞存活率、线粒体活性均显著下降(P<0.01),细胞凋亡率显著升高(P<0.01);13-MTD10、20、40μg/mL可显著提高细胞存活率(P<0.01);13-MTD5、10、20μg/mL可显著下调细胞凋亡率(P<0.01);13-MTD5、10、20、40μg/mL可显著提高细胞MTT代谢率(P<0.01);且存在一定量效差异(P<0.05,P<0.01)。结论:不同剂量的13-MTD对H2O2诱导的SH-SY5Y神经细胞氧化损伤具有保护作用。

AIM：To investigate the protective effects of 13-methyltetradecanoic acid（13-MTD） on the injury of cultured SH-SY5Y neural cells induced by hydrogen peroxide（H2O2）.METH-ODS：The injury of cultured SH-SY5Y cells was made in vitro by H2O2.Microscope was used to detect the morphology of cells.Sulforhodamine B assay was applyed to detect the cell viability.Acridine orange/ethidium bromide （AO/EB） double staining was adopted to observe the apoptotic morphology.MTT assay was employed to detect the mitochondrial reducibility.RESULTS：The cells apoptosis rate was increased,the cells viability and mitochondrial reducibility were decreased significantly in group model of H2O2 injury compared with the group control （P〈0.01）.13-MTD 10,20,40 μg/mL increased cell survival and 13-MTD 5,10,20 μg/mL reduced the apoptosis rate as well as 13-MTD 5,10,20,40 μg/mL increased the cell MTT metabolic rate compared with the group model of H2O2 injury（P〈0.01）,there are some dose-dependent relationship （P〈0.05,P〈0.01）.CONCLUSION：Different doses of 13-MTD shows the neuroprotection effects and can protect SH-SY5Y cells from the injury induced by H2O2.