奥美拉唑对大鼠中毒性急性胃黏膜损伤的保护作用及机制

Protective effects of Omeprazole on the toxic acute gastric mucosal lesion in rats

目的:研究不同剂量奥美拉唑对大鼠中毒性急性胃黏膜损伤的保护作用,探讨其保护机制。方法:40只SD大鼠随机分成空白对照组,急性中毒对照组,小剂量奥美拉唑治疗组(10mg/kg)和大剂量奥美拉唑治疗组(50mg/kg)。造模后计算溃疡指数,测定血浆内皮素(ET)、血清肿瘤坏死因子α(TNF-α)及胆碱酯酶活力,镜下观察胃黏膜病理改变。结果:成功建立急性胃黏膜损伤动物模型;在此基础上采用奥美拉唑治疗可改善中毒大鼠血液相关检测指标,显著改善胃黏膜溃疡指数(UI)及组织病理情况;且大剂量奥美拉唑组的疗效优于小剂量组(P<0.05)。结论:奥美拉唑治疗能够改善大鼠口服有机磷农药中毒所致的急性胃黏膜损伤,大剂量奥美拉唑治疗的效果更明显;ET、TNF-α不但可作为急性中毒性胃黏膜损伤时早期血液检测指标,而且可作为治疗急性中毒性胃黏膜损伤效果的监测指标。

AIM：To study the protective effects of different doses Omeprazole on the toxic acute gastric mucosal lesion in rats.Meanwhile,the protective effects were investigated.METHODS：Forty rats were separated into blank control group,acute intoxication control group,low dose omeprazole treatment group,high dose omeprazole treatment group.Ulcer index was calculated,plasma ET,serum TNF-α and cholinesterase activity were measured.With gastric mucosa HE staining,gastric mucosa pathological changes were observed also.RESULTS：A toxic acute gastric mucosal lesion model were established on rats.Omeprazole could prevent rats from acute gastric mucosal lesion induced by poisoning,improve related indexes and pathological states as well;large dose omeprazole could be more beneficial for acute gastric mucosal lesion induced by poisoning.CONCLUSION：Administration of Omeprazole could improve the acute gastric mucosal lesion induced by orgnaophosphorus pesticide orally in rats,and large dose Omeprazole could be more beneficial;ET and TNF-α in serum could be considered as the surrogate for treatment of acute poisoning gastric mucosal lesion induced as well as index for early detection.