miR-143和miR-145在胃间质瘤组织中的表达及其意义

Clinical significance of miR-143 and miR-145 expression in gastric gastrointestinal stromal tumor

目的 探讨miR-143和miR-145在胃间质瘤发生发展中的作用.方法 采用茎环即时RT-PCR方法检测21例胃间质瘤及其正常胃组织中miR-143和miR-145的表达,分析其与临床病理因素的关系.结果 本组胃间质瘤组织中miR-145表达显著高于正常胃组织（P＜0.01）,且核分裂数≥5/50 HPF病例的miR-145表达显著低于核分裂数＜5/50 HPF病例（P=0.02）,巨大肿瘤（直径＞10 cm）的miR-145表达显著低于大肿瘤（5～10 cm）病例及小肿瘤（2～5 cm）病例（P=0.048）,Fletcher分级高危病例的miR-145表达显著低于中危及低危分级病例（P=0.048）,低危组与中、高危组miR-145表达相比差异有统计学意义（P=0.01）.胃间质瘤组织中miR-143表达与正常胃组织相比差异无统计学意义（P=0.06）. 结论 miR-145在胃间质瘤组织中表达上调,且与肿瘤大小、核分裂象及Fletcher分级等密切相关,提示其在胃间质瘤的发生发展过程中发挥重要作用.

Objective To evaluate the role of miR-143, miR-145 in the development of gastric gastrointestinal stromal tumor. Methods The expression levels of miR-143 and miR-145 in 21 cases of gastric gastrointestinal stromal tumor and the matched non-tumor adjacent tissue specimens were examined by stem-loop real-time RT-PCR, and its correlation with clinicopathologic features of gastric gastrointestinal stromal tumor were analyzed. Results Expression level of miR-145 were significantly higher in tumor than adjacent normal tissues （P〈0.01 ） and that with mitotic count ≥ 5/50HPF cases was significantly lower than that with mitotic count 〈5/50HPF cases （P=0.02）. miR-145 expression in huge tumor （〉10 cm）was significantly lower than that in the large tumor （5～10 cm） and small tumor （2～5 cm） （P=0.048）.By Fletcher risk stratification system, miR-145 expression in high-risk cases was significantly lower than that in the intermediate-risk and low-risk cases （P=0.048）. While the expression of miR-145 in low-risk group was significantly different compared to that in intermediate-risk group and high-risk group （P=0.01）.There was no difference between the expressions of miR-143 in tumor and that in normal tissue（P=0.06）.Conclusion In gastric gastrointestinal stromal tumor, MiR-145 expression is significantly higher in tumor than adjacent normal tissues. miR-145 is closely associated with tumor size. mitotic counts and Fletcher risk stratification system.