外源性磷酸肌酸对偏侧咀嚼大鼠咬肌组织影响的研究

A preliminary study on the effects of the exogenous creatine phosphate on rat masseter muscle after unilateral chew

目的研究能量治疗对偏侧咀嚼大鼠咬肌组织中Ca2+、Ca2+-ATP酶活性的影响,探讨外源性能量物质磷酸肌酸(CP)对偏侧咀嚼大鼠咬肌组织的保护作用。方法将20只大鼠随机分为4组,分别记为A组:磷酸肌酸钠对照组;B组:磷酸肌酸钠实验组;C组:生理盐水对照组;D组:生理盐水实验组。采用原子吸收分光光度法测定Ca2+质量分数;运用Ca2+-ATP酶试剂盒测定Ca2+-ATP酶活性;透射电镜观察咬肌组织超微结构的变化。结果 1)D组拔牙侧Ca2+质量分数较其非拔牙侧(P=0.007)、C组(P=0.009)、B组拔牙侧(P=0.01)均有明显升高;2)D组拔牙侧Ca2+-ATP酶活性较其非拔牙侧(P=0.001)、C组(P=0.003)、B组拔牙侧(P=0.001)均有明显降低;3)透射电镜下观察到B组拔牙侧咬肌组织超微结构与正常对照组相近,而D组拔牙侧咬肌组织超微结构与正常对照组相差较大,损伤表现拔牙侧重于非拔牙侧。结论外源性能量物质磷酸肌酸钠对偏侧咀嚼大鼠咬肌组织有一定的保护作用,有可能成为改善咀嚼肌损伤的一个新途径。

Objective To study the effect of energy therapy on Ca^2＋ concentration and Ca^2＋-ATP enzyme activity in rat master muscle after unilateral chew,and to discuss the protective action of the exogenous creatine phosphate on rat masseter muscle after unilateral chew.Methods The 20 rats were randomly divided into 4 groups,A：Creatine phosphate normal control group;B：Creatine phosphate experimental group;C：Saline normal control group;D：Saline experimental group.The Ca^2＋ concentration were determined by atomic absorption spectrophotometry,the activity of the Ca^2＋-ATP enzyme were determined by super-micro volume Ca^2＋-ATP enzyme kit.Results 1）The Ca^2＋ concentration of the extraction side of group D which received the saline injection had significant difference compared with the non-extraction side（P=0.007）,the group C（P=0.009） and the extraction side of group B（P=0.01）;2）Ca^2＋-ATP enzyme activity of group D were higher than its non-extraction side（P=0.001）,group C（P=0.003） and the extraction side of group B（P=0.001）;3）The ultrastructural changes of the rat masseter muscle under transmission electron microscope were as follows：The extraction side of group D have more severe pathological manifestations than non-extraction side.Both the extraction side and the non-extraction side of group B had a similar manifestation to the normal control group.Conclusion Exogenous energy material,creatine phosphate,may have certain degree of protective effect on rat masseter muscles after unilateral chew.And it may become a possible way to improve the injury of the masseter muscle.