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酸性微环境对单核/巨噬细胞分泌血管内皮细胞生长因子及Tca8113细胞杀伤作用的影响 被引量：4

Experimental study on the anti-tumor effect of monocytes/macrophages against Tca8113 cells and the secretion of vascular endothelial growth factor in acid microenvironment
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摘要 目的通过酸性微环境下单核/巨噬细胞分泌血管内皮细胞生长因子(VEGF)及对舌癌细胞株Tca8113杀伤作用的研究,探讨肿瘤内浸润的单核/巨噬细胞在酸性微环境影响下的功能状态。方法从健康人的外周血中分离单核细胞并通过培养将其转化为单核/巨噬细胞,分别调整培养液的pH值为酸性:6.6、6.8;常规环境:pH值为7.2,将单核/巨噬细胞和舌癌细胞株Tca8113进行混合培养,MTT法检测单核/巨噬细胞对舌癌细胞的杀伤作用;采用ELISA法检测不同微环境下单核/巨噬细胞VEGF的分泌情况。结果在酸性微环境下,单核/巨噬细胞对舌癌细胞株的杀伤作用低于常规环境下(P<0.05);而分泌VEGF的能力较常规环境下增强(P<0.05)。结论可能正是肿瘤组织局部的酸性微环境改变了单核/巨噬细胞的功能,更多的参与了肿瘤的生长,但其在肿瘤中的具体机制及如何调节单核/巨噬细胞在肿瘤组织中的功能还需进一步的研究。 Objective To investigate the anti-tumor effect of monocytes/macrophages against Tca8113 cells and the secretion of vascular endothelial growth factor（VEGF） in acid microenvironment in vitro.Methods Peripheral blood mononuclear cells were extracted from healthy person＇ s blood and cultivated to transform into monocytes/macrophages.The monocytes/macrophages were cultured with Tca8113 in acid microenvironment（pH6.6 and pH6.8） and in normal microenvironmen（tpH7.2）.The anti-tumor effect of monocytes/macrophages against Tca8113 cells were examined by MTT assay.The expression of VEGF was detected by enzyme-link immunoassay（ELISA）.Results The anti-tumor effect of monocytes/macrophages against Tca8113 cells in acid environment was lower than in normal environment（P〈0.05）.VEGF excreted by mononuclear/macrophage was significantly higher in acid microenvironment than in normal microenvironment.Conclusion Due to acid microenvironment inside tumor,the anti-tumor effect of monocytes/macrophages against tumor cell was decreased,but the secretion of VEGF was gradually increased.However,the function of monocytes/macrophages on anti-tumor need more research.
作者 宋宇峰 冯红超 唐路 彭江帆 马洪
机构地区 贵阳医学院口腔医学系
出处 《华西口腔医学杂志》 CAS CSCD 北大核心 2010年第4期364-366,共3页 West China Journal of Stomatology
基金 国家自然科学基金资助项目(30660200) 贵州省优秀科技教育人才省长专项基金资助项目[(2005)160号]
关键词 单核/巨噬细胞 舌鳞状细胞癌 酸性 血管内皮细胞生长因子 monocytes/mcrophages tongue squamous cell carcinoma acid vascular endothelial growth factor
分类号 R739.86 [医药卫生—肿瘤]
  • 相关文献

参考文献9

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共引文献6

同被引文献34

  • 1冯红超,宋宇峰,温玉明.口腔鳞癌中VEGF表达和肿瘤相关巨噬细胞在血管生成中相互作用的初步研究[J].中国肿瘤临床,2004,31(8):435-437. 被引量：8
  • 2宋宇峰,冯红超,温玉明.口腔鳞状细胞癌中巨噬细胞浸润和肿瘤生长、转移的关系[J].中华口腔医学杂志,2005,40(5):385-385. 被引量：9
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引证文献4

二级引证文献9

华西口腔医学杂志
2010年 第4期

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