成人牙周炎不同临床状态牙龈组织中白细胞介素-10mRNA的表达

The expression of interleukin-10 mRNA in gingival lesion of different clinical states in patients with adult periodontitis

目的探讨白细胞介素-10(IL-10)mRNA在活动期和稳定期牙周炎牙龈组织中的表达状况。方法选择急性牙周脓肿患者12例、牙周炎基础治疗后稳定期患者12例及阻生第三磨牙拔除患者6例为研究对象,分别设为试验A组(活动期组)、试验B组(稳定期组)和对照组,采集牙龈组织样本,采用原位杂交技术检测3组样本中IL-10mRNA的表达,并进行半定量分析。结果 IL-10mRNA表达阳性的细胞类型主要有淋巴细胞、巨噬细胞及牙龈成纤维细胞。试验A组IL-10mRNA的表达量最低,明显低于对照组和试验B组(P<0.01);试验B组IL-10mRNA的表达量最高,高于对照组和试验A组(P<0.01)。结论 IL-10mRNA表达量与牙周炎的临床状态密切相关。

Objective To investigate the expression of interleukin-10（IL-10） mRNA in gingival tissue of active and stable stage in patients with adult periodontitis.Methods 12 patients with acute abscesses of the periodontium,12 patients after periodontal initial treatment and 6 periodontal healthy patients having extraction of impacted wisdom tooth were randomly divided into group A（active stage group）,group B（stable stage group） and the control group.Biopsies of gingival tissues were collected from every subject of three groups.Technique of in situ hybridization was applied to observe the expression of IL-10 mRNA in the biopsies from three groups semi-quantitatively.Results IL-10 mRNA was positively expressed in lymphocytes,macrophages and fibroblasts.The quantity of IL-10 mRNA of group A was the lowest in the three groups and was significantly lower than that of control group and group B respectively（P〈0.01）.The quantity of IL-10 mRNA of group B was the highest in the three groups and was significantly higher compared with the control group and group A（P〈0.01）.Conclusion The quantities of IL-10 mRNA expression are closely related with various clinical states of periodontitis.