酪氨酸激酶A和血管内皮生长因子受体2在涎腺腺样囊性癌侵袭转移中的作用

Tyrosine kinase A and vascular endothelial growth factor receptor 2 in the invasion and metastasis of salivary adenoid cystic carcinoma

目的通过检测酪氨酸激酶A(TrkA)与血管内皮生长因子受体2(VEGFR2)在涎腺腺样囊性癌(SACC)的表达情况,探讨TrkA与VEGFR2在SACC侵袭转移中的作用。方法采用免疫组织化学SP法检测47例SACC患者组织病理切片中TrkA与VEGFR2的表达情况,结合临床资料统计分析,评价TrkA、VEGFR2与SACC侵袭转移特性的相关性。结果 TrkA、VEGFR2在SACC组织中的阳性率分别是87.23%(41/47例)和85.11%(40/47例),在有神经侵袭、有复发/转移的SACC患者组织切片中TrkA和VEGFR2的表达率均高于无神经侵袭、未复发/转移者,且差别有统计学意义(P<0.05)。组织内微血管密度(MVD)计数与VEGFR2的表达率成正相关关系;MVD在神经侵袭组为25.14±2.83,在无神经侵袭组为18.81±1.33,两者差异有统计学意义(P<0.05);MVD在复发/转移组为26.58±2.38,未复发/转移组为19.06±1.39(P<0.05)。结论 TrkA、VEGFR2的表达强弱与SACC的嗜神经侵袭,复发转移成正相关关系,提示该2种受体在SACC的侵袭转移过程中具有重要作用,并据此推测TrkA、VEGFR2可以作为评价涎腺腺样囊性癌患者预后的指标。

Objective To determine the effect of tyrosine kinase A（TrkA） and vascular endothelial growth factor receptor 2（VEGFR2） in the invasion and metastasis of salivary adenoid cystic carcinoma（SACC）.Methods The expression of TrkA and VEGFR2 were detected by immunohistochemical staining in 47 cases of SACC of salivary glands.Clinical data were reviewed by multivariate prognostic analysis.Results The positive rate of TrkA and VEGFR2 in SACC was 87.23%（41/47） and 85.11%（40/47） respectively.Express of TrkA and VEGFR2 in perineural invasion and recurrence group were higher than non-perineural invasion and non-recurrence group.Significant difference was found in microvessel density（MVD） and VEGFR2 expression within different groups（P〈0.05）.MVD in perineural invasion group（25.14±2.83） was significantly higher than that in none perineural invasion group（18.81±1.33）（P〈0.05）.MVD in recurrence or metastasis group（26.58±2.38） was significantly higher than tha（t19.06± 1.39） in none recurrence nor metastasis group（P〈0.05）.Conclusion Positive correlation between expression of TrkA,VEGFR2 and nerve invasion and vessel metastasis of SACC indicate that TrkA and VEGFR2 play important roles in the invasion and metastasis of SACC.It is possible that TrkA and VEGFR2 could be an aid for evaluating the prognosis of SACC patients.