不同品种牡丹对丛枝菌根真菌群落结构的影响

Effects of different peony cultivars on community structure of arbuscular mycorrhizal fungi in rhizosphere soil.

对山东菏泽赵楼牡丹园栽培的不同品种牡丹(Paeonia suffruticosa)根围丛枝菌根(arbuscular mycorrhiza,AM)真菌孢子密度、种属构成、种丰度、分布频度、物种多样性指数及其物种组成相似性等进行了研究.结果表明:不同品种牡丹根围AM真菌的种属构成、种丰度和分布频度等均不相同,其中,从‘凤丹’和‘赵粉’根围中各分离到10种AM真菌,从‘乌龙捧盛’和‘洛阳红’根围中分离到9种,从‘胡红’根围中分离到8种;‘凤丹’根围AM真菌孢子密度最高,为59个.50 g-1土,‘胡红’最低,为47个.(50 g)-1土;‘赵粉’的物种多样性指数最高(1.89),‘胡红’最低(1.71).‘凤丹’和‘胡红’的菌根侵染率最高,为63.6%,‘乌龙捧盛’最低,为52.7%.不同牡丹品种之间AM真菌种类组成的相似性系数在0.71～0.95,其中‘乌龙捧盛’和‘凤丹’的相似性系数最高(0.95),而‘胡红’与‘洛阳红’的相似性系数最低(0.71).牡丹基因型能改变AM真菌群落结构特征.

This paper studied the community structure of arbuscular mycorrhizal（AM） fungi in the rhizosphere soil of different peony（Paeonia suffruticosa） cultivars grown in Zhaolou Peony Garden of Heze in Shandong Province.A number of parameters describing this community structure,e.g.,spore density,species-and genera composition,species richness,distribution frequency,species diversity indices,and Sorenson＇s similarity coefficient,were examined.The speciesand genera composition,species richness,and distribution frequency of AM fungi in rhizosphere soil varied with planted peony cultivars.A total of 10 AM fungal species were isolated from the rhizosphere soil of cultivars ＇Fengdan＇ and ＇Zhaofen＇,9 species from the rhizosphere soil of ＇Wulong pengsheng＇ and ＇Luoyang red＇,and 8 species from the rhizosphere soil of ＇Hu red＇.The spore density was the highest（59 per 50 g soil） in the rhizosphere soil of ＇Fengdan＇,but the lowest（47 per 50 g soil） in the rhizosphere soil of ＇Hu red＇;the species diversity index was the highest（1.89） in the rhizosphere soil of ＇Zhaofen＇,but the lowest（1.71） in the rhizosphere soil of ＇Hu red＇;and the mycorrhizal colonization rate was the highest（63.6%） in rhizosphere soil of ＇Fengdan＇ and ＇Hu red＇,but the lowest（52.7%） in the rhizosphere soil of ＇Wulong pengsheng＇.The Sorenson＇s similarity coefficient of AM fungal species composition in the rhizosphere soil among the test cultivars ranged from 0.71 to 0.95,being the highest between ＇Wulong pengsheng＇and ＇Fengdan＇,and the lowest between ＇Luoyang red＇ and ＇Hu red＇.It was concluded that the gene type of peony could change the community structure of AM fungi in rhizosphere soil.