鸡马立克氏病肾肿瘤p15基因的甲基化特异性PCR检测

Methylated specific PCR detection of p15 gene in kidney tumor of chicken with Marek's disease

选取300只1日龄公鸡,人工复制鸡马立克氏病(MD)模型,随机分为对照组和MD组,分别于35日龄、40日龄、45日龄采取鸡肾,观察病理组织学变化,通过甲基化特异性PCR方法,检测了MD病鸡肾肿瘤发生和发展过程中p15基因甲基化的变化,以探讨p15基因甲基化与肾肿瘤发生、发展的关系。结果显示,MD组鸡的临床表现以及肾组织的病理变化符合MD的特征,说明人工复制MD模型成功。在正常鸡肾组织中没有发生p15甲基化,在MD病鸡肾组织中p15甲基化的发生率高达61.7%,与正常对照组差异极显著(P<0.01),并呈时间效应。证实,p15基因甲基化与MD的发生、发展密切相关。

To investigate the relationship between the methylation of p15 gene and the genesis of kid-ney tumor in the development of Marek＇s disease（MD）, 300 one-day-old chicks were randomly divided into control group and MD group. On 35,40 and 45 days-old, kidneys were collected and then pathologic changes were observed in the experimental replication MD cases and the variations of methylation of p15 gene in the genesis and development of kidney tumor of chicks with MD were detected by the methylated specific PCR. The results showed that the clinical manifestations and pathological changes of the kidney in the MD group were in accord with characteristics of MD,indicating that MD model was successfully generated. No methy-lation of p15 gene was generated in the control group,while 61.7% of methylation of p15 gene was found in the MD group, which was significantly higher（P〈0.01） than that in the control group in time-dependent manner. It was concluded that the methylation of p15 gene was intimately relevant to the genesis and development of MD.