抗β1肾上腺素受体自身抗体的致心律失常效应

The proarrhythmic effects of autoantibodys against β_1 adrenergic receptor

目的:观察抗β1肾上腺素受体自身抗体(β1AA)在心律失常患者血清中的分布特征并探究该抗体是否具有致心律失常作用。方法:选择临床各型心律失常、冠心病患者和正常健康体检者,采用酶联免疫吸附法(ELISA)测定血清中β1AA的滴度;提纯抗体阳性患者血清中的IgG抗体,给予正常大鼠,动态监测心电图的变化,观察心律失常发生频率。结果:β1AA在心律失常患者血清中的阳性率为52.8%,明显高于冠心病对照组(24.0%,P<0.01)与正常对照组(5.0%,P<0.01);β1AA可引发正常大鼠发生心律失常,其中以室性心律失常多见。结论:β1AA在心律失常患者血清中的分布呈高阳性率,并可致大鼠发生心律失常。

Objective： To investigate the distribution characteristics of autoantibody against β1 adrenergic receptor （β1AR） in the sera of arrhythmia patients and whether the autoantibody could induce arrhythmia. Methods： Healthy subjects and patients with arrhythmia or coronary artery disease were chosen. The autoantibody against β1AR in the sera was screened by enzyme-linked immunosorbent assay （ELISA）. IgG in the positive autoantibody sera from arrhytMfia patients were purified and administrated to normal rats; then the ECGs were dynamic monitored. Results： The positive rate of autoantibody against β1AR in arrhythmia patients was 52.8%, which was significantly higher than that in coronary heart disease group （24%, P 〈 0.01） and healthy people group （5%, P 〈 0.01 ）, respectively. Moreover, the autoantibody against β1AR could lead to the occurring of arrhythmia in normal rats, most of which were ventricular arrhythmia. Conclusion： In the sera of arrhythmia pa- tients, the autoantibody against β1 AR has a high titer and it could lead to the arrhythmia of rats in vivo.