复方丹参对急性汞中毒家兔的保护作用及其机制

Protective effects and pathogenesis of complex salvin miltiorrhiza on acute mercury poisoning in rabbits

目的:探讨复方丹参对急性汞中毒家兔的保护作用及其机制。方法:采用皮下注射1%氯化高汞(Hg-Cl2,1.7ml/kg)溶液,复制家兔急性汞中毒的动物模型。动态观察血尿素氮(BUN)、血浆铜蓝蛋白(CP)、乳酸脱氢酶(LDH)、酸性磷酸酶(ACP)、丙二醛(MDA)及红细胞超氧化物歧化酶(SOD)含量的变化;检测肾皮质、肺、肝、心及脾脏组织匀浆MDA、SOD的含量;同时检测尿蛋白和尿沉渣;并与丹参处理组和生理盐水对照组比较。结果:注射HgCl2后0.5h和9.5h分别给予复方丹参注射液2ml/kg,可使BUN、CP、LDH、ACP及MDA含量降低,且可防止SOD减少,各项指标与汞中毒组比较均有统计学意义(P<0.05,P<0.01);尿蛋白和尿沉渣中的管型也明显减少。结论:急性汞中毒后不仅损害肾脏,而且累及多个器官组织。复方丹参能通过稳膜作用减轻或在一定程度上防止急性汞中毒过程的进行性加剧。

Objective： To study protective effect and pathogenesis of complex salvia mihiorrhiza（DanShen）on acute mercury poisoning in rabbits. Methods： Models of acute mercury poisoning was made in rabbits. The effect of complex salvia miltiorrhiza on blood urea nitrogen （BUN）, copper-protein（CP）, lactate dehydrogenase（LDH）, acid phosphatase（ACP）and the malondialdehyde （MDA） in plasma, superoxide dismutase（SOD） in erythrocyte and MDA , SOD in tissues homogenate were observed. Results： The administration of complex salvia mihiorrhiza after mercury injection 0.5 h and 9.5 h, decreased BUN, CP, MDA, LDH and ACP, and prevented the reduction of SOD. Compared with mercury poisoning group, the difference was statistical significant（ P 〈 0.05, P 〈 0.01 ）. Conclusion： It is suggested that acute mercury poisoning may result in renal damage but also multiple organ tissues, and complex salvia miltiorrhiza possesses protective effect, through stabilized membrans.