平行检测血清和尿液中循环抗原诊断日本血吸虫病的研究

DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA BY PARALLEL TESTINGCIRCULATING ANTIGENS IN SERUM AND URINE

应用ＭｃＡｂ－Ｄｏｔ－ＥＬＩＳＡ平行检测血吸虫病人的血清和尿样（浓缩２０倍）中日本血吸虫肠相关趋阴极循环抗原（ＣＣＡ）。８１例急性血吸虫病患者的血清和尿样的阳性率分别为９６．３０％和６６．６７％，而血清和尿样组合检测的阳性检出率提高至１００％。１０９例慢性血吸虫病患者的血清和尿样的阳性检出率分别为７７．０６％和１０．０９％，组合检测的阳性检出率没有提高。１００份健康人的血清和尿样的假阳性率均为０％。５４例华支睾吸虫病患者的血清和尿样的交叉反应率分别为９．４３％和０％。４８例钩虫病患者的血清和尿样的交叉反应率均为０％。

The levels of schistosome circulating cathodic antigen (CCA) in serum and urine were detected parallelly by McAbDotELISA. In 81 acute cases, the positive rates in serum and urine were 96.30% and 66.67% (P<0.01) but the combined positive (positive in serum and/or in urine) rate reached 100%, while in 109 chronic cases, they were 77.06% and 10.09% (P<0.01) but the combined positive rate was not increased. In 100 healthy individuals from nonendemic areas, no positives were detected in serum and urine with false positive rate of 0%. The cross reaction rates in serum and urine of 51 cases with clonorchiasis were 9.43% and 0% but all were 0% in 48 cases with ancylostomiasis.