摘要
目的:研究甲异靛对人慢性粒细胞白血病细胞系K562细胞的影响,探讨甲异靛治疗慢性粒细胞白血病(CML)的作用机理。方法:应用剂量反应曲线,台盼蓝拒染实验,细胞形态学,DNA电泳,流式细胞仪及TUNEL标记等多种方法观察甲异靛对K562细胞的作用。结果:甲异靛能明显抑制K562细胞的增殖,并同时诱导K562细胞凋亡。结论:甲异靛治疗CML的机理可能与甲异靛引起白血病细胞受抑和细胞发生凋亡有关。
Objective: To study the effect of meisoindigo on human chronic myelogenous leukemia (CML) cell line K562 cells for exploring the mechanism of meisoindigo in treatment of CML. Methods: Multiple methods, including dose response curve, trypan blue exclusion, cytomorphology, DNA electrophoresis, flow cytometry and TUNEL (TdT mediated dUTP nick and labeling), were used to observe the effect of meisoindigo on K562 cells. Results: Meisoindigo can inhibit the proliferation and induce the apoptosis of K562 cells. Conclusion: The therapeutic mechanism of meisoindigo in treating CML may be related with its proliferation inhibiting and apoptosis inducing effect in CML cells.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
1999年第6期353-355,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
卫生部课题基金
关键词
甲异靛
慢性
白血病
细胞凋亡
meisoindigo, chronic myelogenous leukemia, apoptosis