茶多酚对奥氮平诱致肥胖大鼠的减肥及调脂作用

Reducing obesity and regulating serum lipid of tea polyphenols on obesity rats induced by olanzapine

目的探讨茶多酚对抗精神病药奥氮平诱致肥胖大鼠的减肥及调脂作用。方法雌性健康大鼠50只,10只为正常对照组,其余40只灌胃给予奥氮平(1.2mg·kg-1)2周,每间隔2天测定体重和饮食饮水量。将肥胖大鼠随机分为模型组(9只),西布曲明组(2.5mg·kg-1,8只),茶多酚高(300mg·kg-1,8只)、低(150mg·kg-1,8只)剂量组。动物继续给予奥氮平(1.2mg·kg-1)处理,同时灌胃给予上述药物处理5周,药物处理期间每周测定体重1次,测定饮食饮水量2次,并于药物处理结束时取血测定血脂。结果茶多酚处理肥胖大鼠至5周时,摄食量、饮水量和体质量明显低于模型大鼠(P<0.05,P<0.01),Lee’s指数、脂肪重量和脂肪系数均明显低于模型大鼠(P<0.01),明显降低肥胖动物的LDL-C和FFA含量(P<0.05,P<0.01),对TC和TG水平也略有降低作用。结论茶多酚对奥氮平诱导的肥胖大鼠具有降低体重,抑制肥胖,调节血脂的作用,其作用可能与抑制动物摄食量和饮水量,增加动物活动有关。

OBJECTIVE To investigate the reducing obesity and regulating serum lipid of tea polyphenols on obesity rats induced by olanzapine. METHODS Female SD rats were randomly divided into normal congtrol group（10rats）and obesity model group（40rats）which was given olanzapine（1.2mg·kg^-1, q. d. ）via gavage 1 h before the beginning of the dark-phase. Body weight and amount of food and water were measured each 2 days. Then obesity rats were randomly divided into model group（9 rats）, sibutramine group（2.5mg·kg^-1, 8 rats）, tea polyphenols high（300mg·kg^-1, 8 rats） and low（ 150mg·kg^-1, 8 rats）groups. These drugs and Olanzapine（ 1.2mg·kg^-1, q.d. ）were administered to rats for 5 weeks by intragastric administration. During drugs treatment, body weight were measured each time one week and amount of food and water were detected twice one week. Serum lipid（TC, TG, HDL-C, LDL-C, FFA）was measured at experimental end. RESULTS After tea polyphenols treat- ment for 5 weeks, food intake, water drinking and body weight were significantly lower than those of model group （P〈0.05 or P 〈 0.01）; Lee＇s index, fat weight and coefficient of fat were significantly lower than those of model group too（P 〈 0.05 or P 〈 0.01 ） ; the contents of LDL-C and FFA were reduced significantly（ P 〈 0.05 orP〈0.01）, and the levels of TC and TG were decreased too. CONCLUSION Tea polyphenols can reduce body weight, inhibiting obesity and regulating serum lipid in obesity rats induced by olanzapine.These effects may be concerned with inhibiting food intake and water drinking, increasing animal spontaneous activity.