犬用口服狂犬病减毒疫苗返祖试验和猴体安全性试验研究

Ancestor returning test and monkey body s safety test of oral live attenuated rabies vaccine for doge

目的:研究犬用口服狂犬病减毒活疫苗的安全性。方法:10只犬分为5组,用犬脑传代,每代2只进行返祖试验;猴体安全性试验,用高于现场用量4倍浓度疫苗肌肉注射后,采血进行中和抗体检测。结果:传代的1～5代犬剖取脑检查均未发现内基氏小体,各代犬脑组织经11～12g小白鼠脑内注射,亦未分离出病毒;传代保留的5只犬,从第1代开始至第5代观察期最长100天,亦健存,而且无任何不良现象。猴体肌肉注射疫苗后,均健康存活,中和抗体1个月为1:13.8～1:19.5,2个月上升为1:33.5～1:37.6。结论:证实本疫苗对家犬口服免疫后,无致病性,毒力未返祖,亦可刺激机体产生中和抗体,达到免疫效果。

AIM To ensure the safety of oral Live attenuated rabies vaccine for dogs. METHODS Ancestor returning test;five groups,each two dogs using dog's brain go down to posterity; Monkey body's safety test; After muscular injection with foufold concentration vaccine,determined neutralizing antibody in blood. RESULTS No Negri's bodies was found in dog's brain tissue of five generations. No virus was separated from llg to 12g weighing mice's brain injected with dog's brain tissue. Five dogs was left lived healthily in 100 days. After inmmuniaztion monkeies lived healthily, their neutralizing antibody was 1 : 13. 8~1 : 19. 5 in one month,1 : 33. 5~ 1 : 37. 6 in 2 months. CONCLUSION;The immunization for dogs proved that the vaccine had no pathogenicity and it's toxicity didn't treurn to ancestor, and it also produce neutralizing antibody and achieve the efffect of immunity.