口服香菇多糖脂质体激活鼠胃肠淋巴系统自然杀伤细胞及其抑瘤作用

EFFECT OF ORAL LENTINANLIPOSOME ON NK CELLS OF GI LYMPHATICTISSUE AND ITS ANTITUMOR ACTIVITY

目的：为口服生物反应调节剂脂质体阻断某些胃肠道肿瘤的形成及术后转移复发提供实验依据。方法：Ｂａｌｂ／ｃ小鼠腹腔接种Ｈ２２肝癌细胞前后按不同分组分别灌胃香菇多糖脂质体、香菇多糖、空白脂质体＋香菇多糖及生理盐水１９ｄ，结束治疗后检测腹水量和肠系膜淋巴结及脾脏ＮＫ细胞活性。结果：香菇多糖脂质体组腹水量显著减少Ｐ＜０．０１，肠系膜淋巴结及脾脏ＮＫ活性显著提高，香菇多糖和香菇多糖＋空白脂质体组ＮＫ活性略升高，但不能抑制腹水生成。结论：香菇多糖脂质体可显著提高荷瘤小鼠肠系膜淋巴结和脾脏ＮＫ细胞活性，口服这种新型的免疫调节剂对ＮＫ细胞的激活可能为其体内抑瘤作用机制之一，表现了长期口服给药抑瘤优势。

OBJECTIVE :To provide evidence for oral liposomes entrapping bioreaction modifier to block the development of some GI tumor and postoperative metastasis and recurrence. METHODS :Balb/c mice were adopted in the study.They were divided to receive oral lentinan(LNT),lentinanliposome or liposome and lentinan for 19 d. Then the H22 tumor cells were injected for all animals on the fourth day of treatment.The NK activity of lymphoglandulae messentericae and spleen,and the volume of ascites were measured. RESULTS :The NK activity of lymphoglandulae mestericae and spleen,and the volume of ascties were significantly increased after treatment with lentinanliposome. CONCLUSION: The NK activity of lymohoglandulae mesentericae and spleen can be enhanced by oral lentinanliposome which may be an important mechanism of its in vivo antitumor effect.