成骨细胞培养用于防治骨质疏松症新药的筛选

CULTURE OF OSTEOBLAST FOR SIFTING NEW DRUG IN PREVENTION AND CURE OF OSTEOPOROSIS

寻找最适合的用于防治骨质疏松症新药筛选的成骨细胞系。方法：采用不同来源组织培养成骨细胞，分别加入已知对成骨细胞有促骨形成作用的地塞米松和氢化可的松，通过ＶｏｎＫｏｓａ染色，定量表示出骨形成，比较不同组织培养的成骨细胞用于药物筛选的优点和缺点。结果：ＭＣ３Ｔ３Ｅ１细胞最易获得，其次是大鼠胎儿成骨细胞；小鼠成骨细胞培养周期长，成活率低；大鼠大腿骨成骨细胞稳定性不好，培养方法还不成熟；成熟大鼠成骨细胞培养方法成熟。结论：成熟大鼠成骨细胞是用于骨质疏松症相关的骨形成研究和其药物筛选最适宜的成骨细胞系。

OBJECTIVE :To explore the most suitable clone of osteoblast for sifting new drugin prevention and cure of osteoporosis. METHODST]:Dexamethasone and hydrocortisone,agents of wellknown osteogenic action,were added to osteoblast cultured from different sources,then stained with Von Kossa and their advantage and disadvantage were quantitatively shown through comparison with blank. RESULTS :MC3T3E is the osteoblast of most easily to obtain,next is the one cultured from foetus of rat.Osteoblast of mouse has a long period of culture and low survival rate;osteoblast from thigh of rats has low stability with premature method of culture.Osteoblast from adult rats is stable with a mature method of culture. CONCLUSION :The most suitable osteoblast for research in osteogenesis and sifting drug for prevention of osteoporosis is the one cultured from adult rats.